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Invitrogen™
RNAqueous™-Micro Total RNA Isolation Kit
The RNAqueous™-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from less than 500,000 cultured cells,Read more
| Catalog Number | Quantity |
|---|---|
| AM1931 | 50 Preps |
Catalog number AM1931
Price (CNY)
4,253.00
飞享价
Ends: 31-Dec-2025
5,315.00Save 1,062.00 (20%)
Each
Quantity:
50 Preps
Price (CNY)
4,253.00
飞享价
Ends: 31-Dec-2025
5,315.00Save 1,062.00 (20%)
Each
The RNAqueous™-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from less than 500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. This kit provides:
• Highly concentrated RNA—for increased sensitivity in downstream assays
• Isolation of high-quality RNA from micro-scale samples
• Reagents/protocol for RNA recovery from LCM samples
How it works
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 μL. Residual genomic DNA is efficiently removed using the DNA-free™ system included in the kit. The RNAqueous™-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.
• Highly concentrated RNA—for increased sensitivity in downstream assays
• Isolation of high-quality RNA from micro-scale samples
• Reagents/protocol for RNA recovery from LCM samples
How it works
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 μL. Residual genomic DNA is efficiently removed using the DNA-free™ system included in the kit. The RNAqueous™-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elution Volume10–20 μL
Final Product TypeTotal RNA
For Use With (Application)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Purification Time15 min. (After sample homogenization)
Quantity50 Preps
Shipping ConditionBox 1: Dry Ice
Box 2: Room Temperature
Box 2: Room Temperature
Starting Material AmountCells: ≤105
Tissue: ≤10 mg
Tissue: ≤10 mg
Isolation TechnologySilica Spin Column
Sample TypeCells, Tissue, Laser Capture Microdissection (LCM)
Unit SizeEach
Contents & Storage
• 50 Filter Cartridges with Collection Tubes; store at room temperature
• 50 Elution Tubes (1.5 mL); room temperature
• 15 mL Wash Solution 1 (concentrated); room temperature
• 28 mL Wash Solution 2/3 (concentrated); 4°C
• 25 mL Lysis Solution; 4°C
• 200 μL LCM Additive; -20°C
• 55 μL DNase; -20°C
• 600 μL 10X DNase I Buffer; -20°C
• 150 μL DNase Inactivation Reagent; -20°C
• 5 mL Elution Solution; room temperature
• 50 Elution Tubes (1.5 mL); room temperature
• 15 mL Wash Solution 1 (concentrated); room temperature
• 28 mL Wash Solution 2/3 (concentrated); 4°C
• 25 mL Lysis Solution; 4°C
• 200 μL LCM Additive; -20°C
• 55 μL DNase; -20°C
• 600 μL 10X DNase I Buffer; -20°C
• 150 μL DNase Inactivation Reagent; -20°C
• 5 mL Elution Solution; room temperature