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Catalog Number | Unit Size | Price (CNY) | Availability | Quantity | |
---|---|---|---|---|---|
AM8439 | 50 µl | Price: 3,257.00 Online offer: 2,281.00 (ends 31-Dec-2024) Your price: | *** |
When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups. The modified dUTP is readily incorporated by reverse transcriptase. The aminoallyl modification enables downstream reaction with amine-reactive compounds such as N-hydroxysuccinimide (NHS) esters; thus aminoallyl-modified cDNA can be labeled with any amine-reactive group moiety.
Use of this modified nucleotide
Use 5-(3-aminoallyl)-dUTP as recommended in your protocol for reverse transcription. Substitution of 30–80% of the dTTP with 5-(3-aminoallyl)-dUTP may be useful, but the exact proportion will depend on experimental goals.