BCECF, AM (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein, Acetoxymethyl Ester)
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BCECF, AM (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein, Acetoxymethyl Ester)
Invitrogen™

BCECF, AM (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein, Acetoxymethyl Ester)

With a pKa of ∼6.98, the cell-permeant, dual-excitation ratiometric pH indicator BCECF, AM is ideal for measuring changes in theRead more
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Catalog NumberQuantity
B117020 x 50 μg
B11501 mg
Catalog number B1170
Price (CNY)
4,771.00
Each
Add to cart
Quantity:
20 x 50 μg
Price (CNY)
4,771.00
Each
Add to cart
With a pKa of ∼6.98, the cell-permeant, dual-excitation ratiometric pH indicator BCECF, AM is ideal for measuring changes in the cytosolic pH of most cells. Intracellular pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ∼490 nm versus the emission intensity when excited at its isobestic point of ∼440 nm.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypepH Indicator
Quantity20 x 50 μg
Shipping ConditionRoom Temperature
For Use With (Equipment)Microplate Reader
Product TypepH indicator
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C.

Frequently asked questions (FAQs)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.