BacLight™ Bacterial Membrane Potential Kit

The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) andRead more

Catalog Number	Quantity
B34950	1 kit

Catalog number B34950

Price (CNY)

3,570.00

Ends: 31-Dec-2025

4,744.00

Save 1,174.00 (25%)

Add to cart

Quantity:

1 kit

Price (CNY)

3,570.00

Ends: 31-Dec-2025

4,744.00

Save 1,174.00 (25%)

Add to cart

The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC₂(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC₂(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.

View additional information about all microbiology assays for flow cytometry.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

Dye TypeMembrane Potential Probes

FormatTube(s)

Quantity1 kit

Shipping ConditionWet Ice

Emission482/497

For Use With (Application)Membrane Potential Assay

For Use With (Equipment)Flow Cytometer

Product LineBacLight

Product TypeBacterial Membrane Potential Kit

Unit SizeEach

Contents & Storage

Contains DiOC₂(3) (1.2 mL of 3 mM solution in DMSO), CCCP (300 μL of 500 μM solution in DMSO), and PBS (200 mL). Store in refrigerator (2–8°C) and protect from light.

Frequently asked questions (FAQs)

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I look at bacteria and yeast on a flow cytometer?

Yes, you can. We offer:

-Counting assays: Bacteria Counting Kit, for flow cytometry (Cat. No. B7277) or LIVE/DEAD BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856).
-Viability/vitality assays: LIVE/DEAD BacLight Bacterial Viability Kit (Cat. No. L13152).
-Membrane potential: BacLight Bacterial Membrane Potential Kit (Cat. No. B34950).
-Yeast viability/vitality assays: LIVE/DEAD FungaLight Yeast Viability Kit, for flow cytometry (Cat. No. L34952), FungaLight Yeast CFDA, AM/Propidium Iodide Vitality Kit (Cat. No. F34953)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.