The BacLight™ RedoxSensor™ CTC Vitality Kit provides effective reagents for evaluating bacterial cell health and vitality that can withstand fixation procedures. Briefly, healthy cells respiring via the electron transport chain will absorb and reduce CTC into an insoluble, red fluorescent formazan product. Cells not respiring or respiring at slower rates will reduce less CTC and consequently produce less fluorescent product, giving a semi-quantitative estimate of healthy vs. unhealthy bacteria. Green- and blue-fluorescent nucleic acid stains are included as counterstains to assist differentiation of cells from debris and calculating total cell numbers.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell Type
Bacteria
Detection Method
Fluorescent
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer
Product Type
CTC Vitality Kit
Dye Type
Other Label(s) or Dye(s)
Emission
UV, 488
Format
Tube(s), Slide(s)
Product Line
BacLight™, RedoxSensor™
Quantity
50
Shipping Condition
Room Temperature
Solubility
DMSO (Dimethylsulfoxide)
Contents & Storage
Contains 5 vials of 5-cyano-2,3-ditolyl tetrazolium chloride (CTC, 15 mg per vial), 1 vial of SYTO™ 24 green (100 μL in DMSO)m and DAPI (100 μL, 5 mg/mL solution in water). Store in freezer (-5 to -30°C) and protect from lig