BODIPY™ 665/676 (Lipid Peroxidation Sensor)

BODIPY 665/676 is a lipophilic probe used to detect lipid peroxidation. It exhibits a change in fluorescence after interaction with peroxyl radicals within cells. We recommend dissolving BODIPY 665/676 in high-quality anhydrous DMSO to attain a stock concentration of 1 to 10 mM.

Lipid peroxidation is the oxidative degradation of cellular lipids by free radicals from reactive oxygen species, which can degrade lipids containing carbon-carbon double bonds such as phospholipids and polyunsaturated fatty acids. Oxidation of lipids leads to the generation of lipid peroxides and can cause damage to cell membranes, resulting in changes to signal transduction pathways and eventually leading to cell death. Lipid peroxidation is involved in apoptosis and one of the main contributors to ferroptosis, an iron-dependent, non-apoptotic form of cell death. Oxidative stress from lipid peroxidation plays a role in aging, as well as pathologies such as cancer, atherosclerosis, and neurodegenerative diseases.