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Catalog Number | Label or Dye | Reactive Moiety | Molecular Weight (g/mol) |
---|---|---|---|
C20021 | Alexa Fluor™ 555 | sDIBO Alkyne | ∼1800 |
C10407 also known as C-10407 | Alexa Fluor™ 594 | DIBO Alkyne | ∼1200 |
C20020 | Alexa Fluor™ 488 | sDIBO Alkyne | ∼1400 |
C20022 | Alexa Fluor™ 647 | sDIBO Alkyne | ∼1900 |
C20023 | Biotin | sDIBO Alkyne | ∼1000 |
C20025 | SDP ester | SDP ester, sDIBO Alkyne | ∼1050 |
Click-iT sDIBO Alkyne labels react with azides via a copper-free Click chemistry reaction to form a stable triazole linkage with an Alexa Fluor, Biotin, or label of your choice. Copper-free click reactions using our sDIBO alkynes are ideal for surface labeling of live cells; they also minimize damage to fluorescent proteins like GFP or R-PE. For intracellular labeling, the nonspecific binding of DIBO alkynes creates a background signal, and a labeling strategy using picolyl azides is the preferred solution in these instances.
Click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background. Macromolecules that have been azide-modified enzymatically, chemically, or metabolically can be labeled more easily, yielding more soluble bioconjugates with improved biological labeling utility.
To make a concentrated stock solution from 2–5 mM, dissolve the dye-alkyne in high quality anhydrous DMSO. Store frozen, desiccated, and protected from light. Click-iT SDP Ester sDIBO alkyne is susceptible to hydrolysis, so prepare the stock solution fresh on the day of the experiment.