Cholera Toxin Subunit B (Recombinant) Conjugate

Recombinant cholera toxin B conjugates for GM1 labeling and neuronal tracing

Cholera toxin B subunit (CT-B) binds with high affinity to GM1 ganglioside on the cell surface and is widely used for neuronal tracing, GM1 labeling, endocytosis, and membrane trafficking studies. At neutral pH, CT-B forms a pentamer with multiple GM1-binding sites, enabling multivalent interactions with GM1-containing membranes.

These conjugates are made using recombinant cholera toxin B subunit without the enzymatically active A subunit of native cholera toxin.

Why use recombinant cholera toxin B conjugates?

• Label GM1 ganglioside: Use the high-affinity interaction between CT-B and GM1 to visualize and detect GM1-associated membrane structures.
• Trace neuronal projections: Use CT-B for well-established retrograde labeling of neuronal pathways.
• Study membrane trafficking: Follow CT-B/GM1 internalization and retrograde trafficking through endosomal pathways.
• Investigate membrane organization: Label GM1 and examine GM1-associated membrane domains in lipid raft-related studies.
• Use recombinant B subunit: Study GM1 binding and trafficking with CT-B lacking the enzymatically active A subunit of native cholera toxin.
• Choose multiple detection formats: Select fluorescent Alexa Fluor™ conjugates, Biotin-XX, or HRP to match fluorescence, affinity-based, or enzyme-based detection workflows.

Choose a CT-B conjugate for your detection workflow

Select from Alexa Fluor™ 488, 555, 594, and 647 CT-B conjugates for fluorescence imaging, Biotin-XX for avidin- or streptavidin-based detection, or HRP for enzyme-based detection.

Why choose from multiple conjugates?

• Match your imaging system: Select an Alexa Fluor™ conjugate compatible with available excitation sources, emission filters, and detectors.
• Support multicolor imaging: Choose spectrally distinct Alexa Fluor™ dyes for use with other fluorescent probes, antibodies, and cellular markers.
• Enable affinity-based detection: Use Biotin-XX conjugates with avidin- or streptavidin-based detection workflows.
• Enable enzyme-based detection: Use HRP conjugates for chromogenic or chemiluminescent detection workflows.
• Use one GM1-binding probe across workflows: Apply recombinant CT-B with different labels while maintaining the same GM1-binding biological probe.

Applications of cholera toxin B conjugates

Cholera toxin B conjugates are versatile probes for neuronal tracing, GM1 ganglioside labeling, endocytosis, and membrane trafficking studies.

Use CT-B conjugates to:

• Perform neuronal tracing, including widely used retrograde labeling of neuronal projections
• Label GM1 ganglioside on cell membranes
• Visualize GM1-associated membrane domains in lipid raft-related studies
• Study CT-B/GM1 internalization and retrograde trafficking through endosomal pathways
• Perform fluorescence imaging with Alexa Fluor™ 488, 555, 594, or 647 conjugates
• Perform avidin- or streptavidin-based detection with Biotin-XX conjugates
• Perform enzyme-based detection with HRP conjugates

For Research Use Only. Not for use in diagnostic procedures.