Calcium Calibration Buffer Kit #1, zero and 10 mM CaEGTA
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Citations & References (66)
Invitrogen™

Calcium Calibration Buffer Kit #1, zero and 10 mM CaEGTA

The Calcium Calibration Buffer Kits are designed to help determine the dissociation constant (Kd) of fluorescent Ca2+ indicators using aRead more
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Catalog NumberQuantity
C3008MP1 Kit
Catalog number C3008MP
Price (CNY)
4,492.00
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Ends: 31-Dec-2025
6,049.00
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Quantity:
1 Kit
Price (CNY)
4,492.00
飞享价
Ends: 31-Dec-2025
6,049.00
Save 1,557.00 (26%)
Each
Add to cart
The Calcium Calibration Buffer Kits are designed to help determine the dissociation constant (Kd) of fluorescent Ca2+ indicators using a fluorometer or quantitative imaging system. The Kd of an indicator can be calculated from a plot generated by scanning the excitation or emission of the indicator in the presence of eleven different Ca2+ concentrations. Calcium Calibration Kit #1 contains 50 mL of 10 mM K2EGTA and 50 mL of 10 mM CaEGTA. Both solutions contain 100 mM KCl and 30 mM MOPS, pH 7.2 and are prepared in deionized water. These stock solutions can be blended to make buffers having free Ca2+ ranging from 0 μM to 39 μM.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeOther Label(s) or Dye(s)
FormatTube(s), Slide(s)
Quantity1 Kit
Shipping ConditionRoom Temperature
For Use With (Application)Calcium Calibration
For Use With (Equipment)Fluorescence Microscope, Fluorometer
Product TypeCalcium Calibration Buffer Kit
Unit SizeEach
Contents & Storage
Store in refrigerator at 2°C to 8°C

Citations & References (66)

Citations & References
Abstract
Mechanism of collagen activation in human platelets.
Authors:Roberts DE, McNicol A, Bose R
Journal:J Biol Chem
PubMed ID:14981087
The mechanism of collagen-induced human platelet activation was examined using Ca2+, Na+, and the pH-sensitive fluorescent dyes calcium green/fura red, sodium-binding benzofuran isophthalate, and 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. Administration of a moderate dose of collagen (10 microg/ml) to human platelets resulted in an increase in [Ca2+](i) and platelet aggregation. The majority of this ... More
The molecular basis of differential subcellular localization of C2 domains of protein kinase C-alpha and group IVa cytosolic phospholipase A2.
Authors:Stahelin RV, Rafter JD, Das S, Cho W
Journal:J Biol Chem
PubMed ID:12531893
'The C2 domain is a Ca(2+)-dependent membrane-targeting module found in many cellular proteins involved in signal transduction or membrane trafficking. C2 domains are unique among membrane targeting domains in that they show a wide range of lipid selectivity for the major components of cell membranes, including phosphatidylserine and phosphatidylcholine. To ... More
Differential regulation of nuclear and cytosolic Ca2+ in HeLa cells.
Authors:Badminton MN, Campbell AK, Rembold CM
Journal:J Biol Chem
PubMed ID:8940122
'The results reported in this study address the controversial issue that nuclear free Ca2+ ([Ca2+]n) may be regulated independently of cytosolic free Ca2+ ([Ca2+]c). We have measured [Ca2+]n and [Ca2+]c with recombinant aequorin targeted to the nucleus and cytosol in HeLa cells. We found that histamine, ATP, and ionomycin increased ... More
A single optical fiber fluorometric device for measurement of intracellular Ca2+ concentration: its application to hippocampal neurons in vitro and in vivo.
Authors:Kudo Y, Akita K, Nakamura T, Ogura A, Makino T, Tamagawa A, Ozaki K, Miyakawa A
Journal:Neuroscience
PubMed ID:1436506
'We developed a new system to measure the intracellular Ca2+ concentration in the deep region of the central nervous system with a single optical fiber (300 microns in diameter), used for both excitation and detection of the fluorescence of previously loaded fura-2. With this system, a brain region loaded with ... More
Protein and acidosis alter calcium-binding and fluorescence spectra of the calcium indicator indo-1.
Authors:Baker AJ, Brandes R, Schreur JH, Camacho SA, Weiner MW
Journal:Biophys J
PubMed ID:7819496
'The fluorescent indicator indo-1 is widely used to monitor intracellular calcium concentration. However, quantitation is limited by uncertain effects of the intracellular environment on indicator properties. The goal of this study was to determine the effects of protein and acidosis on the fluorescence spectra and calcium dissociation constant (Kd) of ... More
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