Modified Lauryl Tryptose Broth w/MUG, Tryptophan (Dehydrated)

Enumerate presumptive Escherichia coli as well as other coliforms, using the most portable number (MPN) method with Thermo Scientific™ Oxoid™ Modified Lauryl Tryptose Broth w/MUG, Tryptophan (Dehydrated).

The formulation contains 4-methylumbelliferyl-β-D-glucuronide (MUG), which is cleaved by the enzyme β-glucuronide to release 4-methylumbelliferone exhibiting blue green fluorescence. The inclusion of tryptophan acts as a substrate for indole production. Both reactions are characteristic of Escherichia coli and can therefore be used to identify presumptive Escherichia coli.

Enumerate coliform organisms using the most portable number (MPN) method, while enumerating presumptive Escherichia coli by means of a culture technique involving a liquid medium containing MUG with Modified Lauryl Tryptose Broth w/MUG, Tryptophan.

The addition of NaOH in the medium improves fluorescence by increasing the alkalinity of the solution. An increase in fluorescence can also be achieved by increasing the level of MUG added to the base medium.

Coliform organisms ferment lactose to produce gas. This production of gas can be taken as positive for the presence of coliforms.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.