Modified Lauryl Sulphate Tryptose Broth Base (Dehydrated)

Selectively enrich Cronobacter spp. from milk and milk samples using Thermo Scientific™ Oxoid™ Modified Lauryl Sulphate Tryptose Broth Base (Dehydrated). Modified Lauryl Sulphate Tryptose Broth Base was originally described by Guillaume-Gentil¹ et al. for the selective enrichment of Cronobacter spp. The high salt concentration of the medium restricts the growth of other members of Enterobacteriaceae and the combination of vancomycin and sodium lauryl sulphate inhibits the growth of Gram-positive organisms commonly isolated in production environments.

Cronobacter (formerly Enterobacter sakazakii) are Gram-negative rod-shaped members of the Family Enterobacteriaceae that have been implicated in outbreaks of disease in premature infants (neonates), causing sepsis, meningitis, and necrotising enterocolitis. Neurological damage can be permanent, and the death rate is reported to be as high as 40-80%².

Cronobacter spp. have been isolated at low levels from powdered infant formulae, and the organisms’ high tolerance to desiccation provides a competitive advantage in the dry environments of milk powder factories, increasing the risk of product contamination³.

Use Modified Lauryl Sulphate Tryptose Broth Base for Selective Enrichment of Cronobacter spp. from milk and milk samples.

• Selective: high salt concentration and the combination of vancomycin and sodium lauryl sulphate makes the medium selective for Cronobacter
• ISO compliant: conforms to ISO TS/22964:2006⁴

The European Union has specified limits for the levels of Cronobacter spp. in dried infant formulae, dried dietary foods for special medical purposes and food intended for infants below six months of age, in Commission Regulation no 2073/20055. This regulation states, for products placed on the market during their shelf-life, 30 samples of 10g have to be tested, and the food producer has to demonstrate the absence of Cronobacter in each of these samples, using ISO/TS 22964:2006⁴.

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