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ChargeSwitch™ NoSpin Plasmid Micro Kit
The ChargeSwitch NoSpin Plasmid Micro Kit provides centrifuge-free microprep plasmid purification that is not only ideal for automation but isRead more
| Catalog Number | Quantity |
|---|---|
| CS10201 | 96 Preps |
Catalog number CS10201
Price (CNY)
3,472.00
Each
Quantity:
96 Preps
Price (CNY)
3,472.00
Each
The ChargeSwitch NoSpin Plasmid Micro Kit provides centrifuge-free microprep plasmid purification that is not only ideal for automation but is adaptable to the KingFisher Flex Purification System. The novel NoSpin reagent causes bacterial cell flocculation and allows cells to be pelleted without the need for automation bottlenecks like centrifugation. This kit includes all the necessary reagents for 0.5–1 mL overnight culture samples with a typical yield of up to 5 μg and requires the use of a magnetic accessory or automation system such as the KingFisher Flex system.
• Provides centrifuge-free purification
• Avoids introduction of enzymatic contaminants for better results
• Optimized for automation
• Ideal for use on the KingFisher Flex system
Performance improvements
This ChargeSwitch NoSpin Plasmid Micro Kit workflow has been adapted to the KingFisher Flex system to allow for purification of 96 samples in about half the time it takes to perform manually. The use of this automated platform results in better performance of the workflow for increased yield (3-fold), purity, and integrity (supercoiling increased from 50% to 90%) (see figures).
Expected Yield (manual): up to 5 μg
Expected Yield (automated): up to 10 μg
Plasmid DNA size: any size plasmid or cosmid
Number of steps (manual): 30
Number of 96 deep well plates: 7
Total time (manual): 90 min
Automated time (hands on + script): 14 min + 30 min (44 min)
Wash steps: 2
Elution volume (μL): 100
• Provides centrifuge-free purification
• Avoids introduction of enzymatic contaminants for better results
• Optimized for automation
• Ideal for use on the KingFisher Flex system
Performance improvements
This ChargeSwitch NoSpin Plasmid Micro Kit workflow has been adapted to the KingFisher Flex system to allow for purification of 96 samples in about half the time it takes to perform manually. The use of this automated platform results in better performance of the workflow for increased yield (3-fold), purity, and integrity (supercoiling increased from 50% to 90%) (see figures).
Expected Yield (manual): up to 5 μg
Expected Yield (automated): up to 10 μg
Plasmid DNA size: any size plasmid or cosmid
Number of steps (manual): 30
Number of 96 deep well plates: 7
Total time (manual): 90 min
Automated time (hands on + script): 14 min + 30 min (44 min)
Wash steps: 2
Elution volume (μL): 100
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypePlasmid DNA
For Use With (Application)Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput CompatibilityHigh-throughput Compatible (KingFisher Flex)
Prep Scale< 100 μg (Small-Scale) Plasmid DNA
Product LineChargeSwitch
Product TypeNoSpin Plasmid Micro Kit
Quantity96 Preps
Sample TypeBacterial Culture
Shipping ConditionRoom Temperature
TargetPlasmid DNA
FormatKit
Isolation TechnologyChargeSwitch Chemistry
Unit SizeEach
Contents & Storage
All components of the ChargeSwitch™ NoSpin Plasmid Kits are shipped at room temperature. Upon receipt, store as follows: Store ChargeSwitch™ Precipitation Buffer (N5) at 4°C. Mix the RNase I in ChargeSwitch™ Resuspension Buffer (R4) and store the resulting solution at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months if stored properly.
Components:
• ChargeSwitch™ MagnaClear™ Beads
• ChargeSwitch™ Magnetic Beads
• ChargeSwitch™ Resuspension Buffer (R4; 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• ChargeSwitch™ Lysis Buffer (L9)
• ChargeSwitch™ Precipitation Buffer (N5)
• ChargeSwitch™ Wash Buffer (W11)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)