DTNB; Ellman's Reagent, 5,5'-Dithiobis-(2-Nitrobenzoic Acid)
DTNB; Ellman's Reagent, 5,5'-Dithiobis-(2-Nitrobenzoic Acid)
Citations & References (181)
Invitrogen™

DTNB; Ellman's Reagent, 5,5'-Dithiobis-(2-Nitrobenzoic Acid)

DTNB or Elman's reagent can be used to quantitate thiols in proteins, cells and plasma by absorption measurements. It readilyRead more
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Catalog NumberQuantity
D845110 g
Catalog number D8451
Price (CNY)
2,665.00
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Quantity:
10 g
Price (CNY)
2,665.00
Each
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DTNB or Elman's reagent can be used to quantitate thiols in proteins, cells and plasma by absorption measurements. It readily forms a mixed disulfide with thiols, liberating the chromophore 5-merapto-2-nitrobenzoic acid (absorption maximum 410 nm). Only protein thiols that are accessible to this water-soluble reagent are modified.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity10 g
Product TypeElman's Reagent
Unit SizeEach

Citations & References (181)

Citations & References
Abstract
Spatial proximity of Cys113, Cys172, and Cys422 in the metalloactivation domain of the ArsA ATPase.
Authors:Bhattacharjee H,Rosen BP
Journal:The Journal of biological chemistry
PubMed ID:8798705
A general strategy for site-specific double labeling of globular proteins for kinetic FRET studies.
Authors:Ratner V,Kahana E,Eichler M,Haas E
Journal:Bioconjugate chemistry
PubMed ID:12236801
Site-directed mutagenesis provides a straightforward means of creating specific targets for chemical modifications of proteins. This capability enhanced the applications of spectroscopic methods adapted for addressing specific structural questions such as the characterization of partially folded and transient intermediate structures of globular proteins. Some applications such as the steady state ... More
Reactivity of mitochondrial sulfhydryl groups toward dithionitrobenzoic acid and bromobimanes under oligomycin-inhibited and uncoupling conditions.
Authors:Freisleben HJ, Fuchs J, Mainka L, Zimmer G
Journal:Arch Biochem Biophys
PubMed ID:2845867
Thiol reactivity was determined in rat heart mitochondria using chromophores of differing polarities: monobromobimane (MB), dithionitrobenzoate (NbS2), and bromobimane-q (MQ). The purpose of this study is to correlate reaction rates of protein thiols in the mitochondrial membrane with the oligomycin-inhibited and uncoupled states: In all cases investigated the reactivity of ... More
Sulfhydryl groups of rabbit liver arylsulfatase A.
Authors:Waheed A, Van Etten RL
Journal:Biochim Biophys Acta
PubMed ID:2864083
Rabbit liver arylsulfatase A (aryl-sulfate sulfhydrolase, EC 3.1.6.1) monomers of 130 kDa contain two free sulfhydryl groups as determined by spectrophotometric titration using 5,5'-dithiobis(2-nitrobenzoate) and by labeling with the fluorescent probe 5-(iodoacetamidoethyl)aminonaphthalene-1-sulfonic acid. Fluorescence quenching data indicate that the reactive sulfhydryl is present in proximity to one or more tryptophan ... More
Actin can act as a cofactor for a viral proteinase in the cleavage of the cytoskeleton.
Authors:Brown MT, McBride KM, Baniecki ML, Reich NC, Marriott G, Mangel WF
Journal:J Biol Chem
PubMed ID:12191991
'Cytoskeletal proteins are exploited by many viruses during infection. We report a novel finding that actin can act as a cofactor for the adenovirus proteinase (AVP) in the degradation of cytoskeletal proteins. Transfection studies in HeLa cells revealed AVP localized with cytokeratin 18, and this was followed by destruction of ... More
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