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Citations & References (9)
Invitrogen™
EnzChek™ Ultra Amylase Assay Kit
Measures amylase activity or screens amylase inhibitors in a high-throughput format
| Catalog Number | Quantity |
|---|---|
| E33651 | 1 Kit |
Catalog number E33651
Price (CNY)
4,303.00
Each
Quantity:
1 Kit
Price (CNY)
4,303.00
Each
The EnzChek™ Ultra Amylase Assay Kit provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek™ kit contains a starch derivative—the DQ™ starch substrate—that is labeled with BODIPY™ FL dye to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.
EnzChek™ Ultra Amylase Assay Kit Specifications:
- Label (Ex/Em): BODIPY™ FL conjugate (∼502/512 nm)
- Kit contains lyophilized substrate, 10X reaction buffer, substrate solvent, a fluorescent standard, and a detailed protocol
- Sufficient reagents are supplied for 500 assays (using a 100 μL assay volume in a 96-well microplate assay format)
Find Fluorescent Substrates for Other Glycosidases
In addition to the EnzChek™ Ultra Amylase Assay Kit, we offer kits and substrates to measure xylanase, lysozyme, β-galactosidase, and more. Review Detecting Glycosidases—Section 10.2 in the Molecular Probes™ Handbook for more information on these products.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Emission Wavelength512 nm
Excitation Wavelength502 nm
Quantity1 Kit
Shipping ConditionRoom Temperature
Substrate PropertiesMolecular Substrate
Substrate TypeAmylase Substrate
Target EnzymeAmylase
For Use With (Application)Enzyme and Metabolic Activity
For Use With (Equipment)Microplate Reader
Product LineEnzChek
Product TypeEnzChek Assay Kit
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.
Frequently asked questions (FAQs)
What is the excitation/emission maxima of the digestion product from EnzChek Ultra Amylase Assay Kit?
Citations & References (9)
Citations & References
Abstract
Counterselection system for Geobacillus kaustophilus HTA426 through disruption of pyrF and pyrR.
Journal:Appl Environ Microbiol
PubMed ID:22885745
'Counterselection systems facilitate marker-free genetic modifications in microbes by enabling positive selections for both the introduction of a marker gene into the microbe and elimination of the marker from the microbe. Here we report a counterselection system for Geobacillus kaustophilus HTA426, established through simultaneous disruption of the pyrF and pyrR
PAR2 exerts local protection against acute pancreatitis via modulation of MAP kinase and MAP kinase phosphatase signaling.
Journal:Am J Physiol Gastrointest Liver Physiol
PubMed ID:18755806
'During acute pancreatitis, protease-activated receptor 2 (PAR2) can be activated by interstitially released trypsin. In the mild form of pancreatitis, PAR2 activation exerts local protection against intrapancreatic damage, whereas, in the severe form of pancreatitis, PAR2 activation mediates some systemic complications. This study aimed to identify the molecular mechanisms of
Cell therapy for salivary gland regeneration.
Journal:J Dent Res
PubMed ID:21297017
'There are still no effective therapies for hyposalivation caused by irradiation. In our previous study, bone marrow stem cells can be transdifferentiated into acinar-like cells in vitro. Therefore, we hypothesized that transplantation with bone marrow stem cells or acinar-like cells may help functional regeneration of salivary glands. Bone marrow stem
Pancreatic contamination of mesenteric adipose tissue samples can be avoided by adjusted dissection procedures.
Journal:J Lipid Res
PubMed ID:18408254
Mesenteric adipose tissue, located in the mesenterium of the intestines, is believed to play a central role in the development of obesity-related diseases. We have found that mesenteric fat samples harvested from rodents are frequently of poor quality, exhibiting partly degraded RNA. To investigate the background for this observation, we
Scanning the Corynebacterium glutamicum R genome for high-efficiency secretion signal sequences.
Journal:Microbiology
PubMed ID:19246745
Systematic screening of secretion proteins using an approach based on the completely sequenced genome of Corynebacterium glutamicum R revealed 405 candidate signal peptides, 108 of which were able to heterologously secrete an active-form alpha-amylase derived from Geobacillus stearothermophilus. These comprised 90 general secretory (Sec)-type, 10 twin-arginine translocator (Tat)-type and eight