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| Catalog Number | Quantity |
|---|---|
| EEA028 | 96 tests |
The assay utilizes the GPO-PAP method, which involves the hydrolysis of triglycerides to glycerol and fatty acids. Glycerol is then oxidized by glycerol kinase to glycerol-3-phosphate, which is oxidized by GPO and produces hydrogen peroxide. In the presence of 4-aminoantipyrine and phenol, hydrogen peroxide is catalyzed by peroxidase (POD) to produce colored quinones which can be measured at 510 nm to calculate the amount of TG in the sample.
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