DNA Polymerase I (10 U/μL)
DNA Polymerase I (10 U/μL)
Thermo Scientific™

DNA Polymerase I (10 U/μL)

Thermo Scientific DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonucleaseRead more
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Catalog NumberQuantity
EP0041500 units
EP00422,500 units
Catalog number EP0041
Price (CNY)
335.00
飞享价
Ends: 31-Dec-2025
479.00
Save 144.00 (30%)
Each
Add to cart
Quantity:
500 units
Request bulk or custom format
Price (CNY)
335.00
飞享价
Ends: 31-Dec-2025
479.00
Save 144.00 (30%)
Each
Add to cart
Thermo Scientific DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity, and ribonuclease H activity.

Highlights

• Incorporates modified nucleotides (e.g. biotin-, digoxigenin-, aminoallyl-, fluorescently-labeled nucleotides)
• Active in multiple buffers, including restriction enzyme, PCR, and RT buffers

Applications

• DNA labeling by nick-translation in conjunction with DNase
• Second-strand synthesis of cDNA in conjunction with RNase H
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionDNA Polymerase I
PolymeraseDNA Polymerase I
Quantity500 units
Concentration10 U/μL
Unit SizeEach

Frequently asked questions (FAQs)

What are the differences between Thermo Scientific DNA Polymerase I, Klenow Fragment, and Klenow Fragment (exo-)?

Thermo Scientific DNA Polymerase I possesses 5′?3′ DNA synthesis activity, 3′?5′ exonuclease (proofreading) activity, and 5′?3′ exonuclease activity. Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5′?3′ polymerase activity and 3′?5′ exonuclease (proofreading) activity, but lacks 5′?3′ exonuclease activity. Klenow Fragment, exo-, is also the large fragment of DNA polymerase I. It exhibits 5′?3′ polymerase activity, but lacks the 3′?5′ and 5′?3′ exonuclease activities.