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Invitrogen™
IL-8 (CXCL8) Human ProcartaPlex™ Simplex Kit, high sensitivity
The Human IL-8 (CXCL8) High Sensitivity Simplex ProcartaPlex Kit measures IL-8 (CXCL8) protein and is designed to be combinable withRead more
| Catalog Number | Quantity |
|---|---|
| EPXS010-10204-901 | 96 Tests |
Catalog number EPXS010-10204-901
Price (CNY)
-
Quantity:
96 Tests
The Human IL-8 (CXCL8) High Sensitivity Simplex ProcartaPlex Kit measures IL-8 (CXCL8) protein and is designed to be combinable with other High Sensitivity Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.
The ProcartaPlex high-sensitivity immunoassays enable the multiplexed analysis of human and mouse analytes in very low concentrations in various matrices, including serum, plasma, bodily fluids, and cell culture supernatant samples. These high-sensitivity multiplex assays are ideal when sample input is limited, and protein expression is in the low pg/mL range.
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.
• More results per sample—measure up to 80 protein targets in a single 25–50 μL sample
• Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation
The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.
The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.
ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.
ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine).
The ProcartaPlex high-sensitivity immunoassays enable the multiplexed analysis of human and mouse analytes in very low concentrations in various matrices, including serum, plasma, bodily fluids, and cell culture supernatant samples. These high-sensitivity multiplex assays are ideal when sample input is limited, and protein expression is in the low pg/mL range.
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.
• More results per sample—measure up to 80 protein targets in a single 25–50 μL sample
• Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation
The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.
The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.
ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.
ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assay RangeAs determined for Lot 1: 0.217 to 890 pg/mL
Assay SensitivitySee Certificate of Analysis
Bead TypeIL-8 (CXCL8) [27 ]
For Use With (Equipment)Luminex™ Instruments
FormatHigh Sensitivity Simplex Kit
GeneC-X-C motif chemokine ligand 8
Gene AliasGCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1
Gene ID (Entrez)3576
Gene SymbolCXCL8
Product LineProcartaPlex
ProteinInterleukin-8
Protein SubtypeEmoctakin, GCP-1, MDNCF, MONAP, NAP-1
Sample TypeSerum, Plasma, Cell Culture Supernatants
Sample VolumeSerum, Plasma: 25 μL, CCS: 50 μL
Shipping ConditionWet Ice
UniProt IDP10145
CombinabilityCombinable (High Sensitivity Only)
Product TypeHigh Sensitivity Simplex Kit
Quantity96 Tests
Research AreaImmunology, Oncology, Neurobiology, Toxicology, Cardiovascular
SpeciesHuman
Unit SizeEach
Contents & Storage
• 1 vial Capture Beads (50X)
• 1 vial biotinylated Detection Antibody (50X)
• 2 vials Human Standard Mix B - HS (lyophilized)
Store at 2°C to 8°C.
Frequently asked questions (FAQs)
What is the size of the Luminex beads you currently use?
I am interested in performing Luminex assays using BioSource kits, and I have a Luminex xMAP system. Besides the kits and system, what other reagents and equipment will I need?
Do the Luminex beads require special care in handling?
Why would the Luminex acquisition software display "Sample Empty" messages during analysis?
What are the Luminex beads made of?
Citations & References (1)
Citations & References
Abstract
Dynamic and Static Exercises Differentially Affect Plasma Cytokine Content in Elite Endurance- and Strength-Trained Athletes and Untrained Volunteers.
Journal:Front Physiol
PubMed ID:28194116
Extensive exercise increases the plasma content of IL-6, IL-8, IL-15, leukemia inhibitory factor (LIF), and several other cytokines via their augmented transcription in skeletal muscle cells. However, the relative impact of aerobic and resistant training interventions on cytokine production remains poorly defined. In this study, we compared effects of dynamic