FilmTracer™ FM™ 1-43 Green Biofilm Cell Stain
FilmTracer™ FM™ 1-43 Green Biofilm Cell Stain
Invitrogen™

FilmTracer™ FM™ 1-43 Green Biofilm Cell Stain

FM dyes are lipophilic styryl compounds used in a wide variety of studies involving plasma membrane and vesiculation. The water-solubleRead more
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Catalog NumberQuantity
F103171 mg
Catalog number F10317
Price (CNY)
4,996.00
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Ends: 31-Dec-2025
6,772.00
Save 1,776.00 (26%)
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Quantity:
1 mg
Price (CNY)
4,996.00
飞享价
Ends: 31-Dec-2025
6,772.00
Save 1,776.00 (26%)
Each
Add to cart
FM dyes are lipophilic styryl compounds used in a wide variety of studies involving plasma membrane and vesiculation. The water-soluble FM® dyes are virtually nonfluorescent in aqueous media and are believed to insert into the surface membrane where they become intensely fluorescent. FM® 1-43 stain has been used successfully to stain the cell bodies specifically in a complex biofilm mileu, including Pseudomonas aeruginosa, Escherichia coli, Staphylococcus sp., Acidothiobacillus caldus, and Vibrio cholerae.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorGreen
Detection MethodFluorescence
Dye TypeFM 1-43⁄lipid
EmissionVisible
Excitation Wavelength Range472⁄580
For Use With (Equipment)Confocal Microscope, Fluorescence Microscope, Flow Cytometer, Microplate Reader, Fluorescent Imager
FormSolid
Product LineFilmTracer™, Molecular Probes™
Quantity1 mg
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeDye
SubCellular LocalizationCell Membranes, Lipids
Unit SizeEach
Contents & Storage
Store at room temperature; Dessicate; Protect from light

Frequently asked questions (FAQs)

The product sheet for FM 1-43 Dye states that the maximum for excitation is 510 nm and emissions is 626 nm, but the spectrum on the product page shows a peak at 470 nm and 580 nm. Can you clarify what the excitation and emission maxima is for this dye?

FM lipophilic styryl dyes are known to shift their spectral properties upon binding to membranes. These dyes exhibit an appreciable solvent and environmental dependency in both free and membrane-bound forms. The emissions of the membrane-bound dye shifts to a shorter wavelength compared to the emission in the solvent.

In solvent, FM 1-43 Dye (Cat. Nos. T35356, T3163, F35355, F10317) absorbs in the range of fluorescein (~490 nm) and initially emits at around 626-636 nm. However, upon membrane integration, it exhibits emission in the 565-590 nm range. Because this dye exhibits a large Stokes shift, it does not perfectly fit into a green or orange fluorophore profile, but is rather a yellow-fluorescent dye, and may require a long-pass emission filter.

Note: FM 1-43 Dye is often used with typical fluorescein or GFP optical filter sets for biological imaging but is poorly excited through tetramethylrhodamine filters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.