Fluo-4FF, Pentapotassium Salt, cell impermeant

Citations & References (11)

Invitrogen™

Fluo-4FF, Pentapotassium Salt, cell impermeant

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. Fluo-5F, fluo-5N, and fluo-4ff are analogsRead more

Catalog Number	Quantity
F23980 also known as F-23980	500 μg

Catalog number F23980

also known as F-23980

Price (CNY)

4,314.00

Add to cart

Quantity:

500 μg

Price (CNY)

4,314.00

Add to cart

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca²⁺. Fluo-5F, fluo-5N, and fluo-4ff are analogs of fluo-4 with lower Ca²⁺-binding affinity, making them suitable for detecting intracellular calcium levels in the 1 μM to 1 mM range that would saturate the response of fluo-3 and fluo-4. Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipette or microinjection or our Influx™ pinocytotic cell-loading reagent. These indicators are compatible with excitation at 488 nm by argon-ion laser sources, making them useful for confocal microscopy, flow cytometry, and microplate screening applications.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

Calcium Indicator (Cell-Impermeant Salts) Specifications:

Label (Ex/Em of Ca²⁺–bound form): Fluo-4FF (494/516 nm)

Fluorescence intensity increase upon binding Ca²⁺: >100 fold

K_d for Ca²⁺ in buffer: ∼9.7 μM

Exhibit fluorescence increase upon binding Ca²⁺ with little shift in wavelength

Using TPEN to Control Heavy Metal Cations

In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn²⁺, Zn²⁺, Pb²⁺) with substantially higher affinity than Ca²⁺. Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN.

More Choices for Fluorescent Calcium Indicators

We offer a large selection of Molecular Probes™ calcium indicators for use in various experimental scenarios. For more information, review Fluorescent Ca²⁺ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes™ Handbook.

For UV-excitable Ca²⁺ indicators, protein-based Ca²⁺ indicators, conjugates of Ca²⁺ indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg²⁺, Zn²⁺) review Indicators for Ca²⁺, Mg²⁺, Zn²⁺ and Other Metal Ions—Chapter 19 in the Molecular Probes™ Handbook.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

Dye TypeFluorescent Dye-Based

Quantity500 μg

Shipping ConditionRoom Temperature

For Use With (Equipment)Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager

Product TypeCalcium Indicator

Unit SizeEach

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.

Citations & References (11)

Citations & References

Abstract

Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging.

Authors:Yasuda R, Harvey CD, Zhong H, Sobczyk A, van Aelst L, Svoboda K

Journal:Nat Neurosci

PubMed ID:16429133

'To understand the biochemical signals regulated by neural activity, it is necessary to measure protein-protein interactions and enzymatic activity in neuronal microcompartments such as axons, dendrites and their spines. We combined two-photon excitation laser scanning with fluorescence lifetime imaging to measure fluorescence resonance energy transfer at high resolutions in brain ... More

Ca2+/calmodulin-dependent protein kinase II (CaMKII) is activated by calmodulin with two bound calciums.

Authors:Shifman JM, Choi MH, Mihalas S, Mayo SL, Kennedy MB

Journal:Proc Natl Acad Sci U S A

PubMed ID:16966599

'Changes in synaptic strength that underlie memory formation in the CNS are initiated by pulses of Ca2+ flowing through NMDA-type glutamate receptors into postsynaptic spines. Differences in the duration and size of the pulses determine whether a synapse is potentiated or depressed after repetitive synaptic activity. Calmodulin (CaM) is a ... More

Nonlinear [Ca2+] signaling in dendrites and spines caused by activity-dependent depression of Ca2+ extrusion.

Authors:Scheuss V, Yasuda R, Sobczyk A, Svoboda K

Journal:J Neurosci

PubMed ID:16885232

'Spine Ca2+ triggers the induction of synaptic plasticity and other adaptive neuronal responses. The amplitude and time course of Ca2+ signals specify the activation of the signaling pathways that trigger different forms of plasticity such as long-term potentiation and depression. The shapes of Ca2+ signals are determined by the dynamics ... More

Synapse-specific plasticity and compartmentalized signaling in cerebellar stellate cells.

Authors:Soler-Llavina GJ, Sabatini BL

Journal:Nat Neurosci

PubMed ID:16680164

'Here we demonstrate that cerebellar stellate cells diffusionally isolate synaptically evoked signals in dendrites and are capable of input-specific synaptic plasticity. Sustained activity of parallel fibers induces a form of long-term depression that requires opening of calcium (Ca(2+))-permeable AMPA-type glutamate receptors (CP-AMPARs) and signaling through class 1 metabotropic glutamate receptors ... More

Concurrent maturation of inner hair cell synaptic Ca2+ influx and auditory nerve spontaneous activity around hearing onset in mice.

Authors:Wong AB, Jing Z, Rutherford MA, Frank T, Strenzke N, Moser T,

Journal:

PubMed ID:23804089

Hearing over a wide range of sound intensities is thought to require complementary coding by functionally diverse spiral ganglion neurons (SGNs), each changing activity only over a subrange. The foundations of SGN diversity are not well understood but likely include differences among their inputs: the presynaptic active zones (AZs) of ... More

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