Search
Citations & References (5)
Invitrogen™
FluoReporter™ Biotin Quantitation Assay Kit, for biotinylated proteins
The FluoReporter™ Biotin Quantitation Assay Kit provides a sensitive fluorometric assay for determining the number of biotin labels on nucleicRead more
| Catalog Number | Quantity |
|---|---|
| F30751 | 1 Kit |
Catalog number F30751
Price (CNY)
5,005.00
Each
Quantity:
1 Kit
Price (CNY)
5,005.00
Each
The FluoReporter™ Biotin Quantitation Assay Kit provides a sensitive fluorometric assay for determining the number of biotin labels on nucleic acid samples (i.e., cDNA). The assay uses Biotective™ Green reagent, which contains a fluorescent dye that is quenched by ligands occupying the biotin binding sites. In the presence of biotin, the quencher dye ligands are displaced and fluorescence occurs. The fluorescence is proportional to the amount of added biotin. The results can be read with any fluorescence-based microplate reader capable of detecting FITC dye.
Important Features of FluoReporter™ Biotin Quantitation Assay Kits:
• Can detect from 4 to 80 picomoles of biotin in a sample (50-fold higher sensitivity than HABA biotin-binding assay)
• Can be applied to as little as 13 ng of biotin-labeled nucleic acid (more is needed for lower degrees of labeling)
• Excitation/emission maxima of the reagent is 495/519 nm
• Supplied with a biotinylated positive control for standard curve
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Important Features of FluoReporter™ Biotin Quantitation Assay Kits:
• Can detect from 4 to 80 picomoles of biotin in a sample (50-fold higher sensitivity than HABA biotin-binding assay)
• Can be applied to as little as 13 ng of biotin-labeled nucleic acid (more is needed for lower degrees of labeling)
• Excitation/emission maxima of the reagent is 495/519 nm
• Supplied with a biotinylated positive control for standard curve
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Product LineFluoReporter
Product TypeBiotin Quantitation Assay Kit
Quantity1 Kit
Shipping ConditionRoom Temperature
Labeling TargetProteins
Label or DyeBiotin
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
Citations & References (5)
Citations & References
Abstract
Designed auto-assembly of nanostreptabodies for rapid tissue-specific targeting in vivo.
Journal:J Biol Chem
PubMed ID:19850928
Molecular medicine can benefit greatly from antibodies that deliver therapeutic and imaging agents to select organs and diseased tissues. Yet the development of complex and defined composite nanostructures remains a challenge that requires both designed stoichiometric assembly and superior in vivo testing ability. Here, we generate nanostructures called nanostreptabodies by
Actin restricts FcepsilonRI diffusion and facilitates antigen-induced receptor immobilization.
Journal:Nat Cell Biol
PubMed ID:18641640
The actin cytoskeleton has been implicated in restricting diffusion of plasma membrane components. Here, simultaneous observations of quantum dot-labelled FcepsilonRI motion and GFP-tagged actin dynamics provide direct evidence that actin filament bundles define micron-sized domains that confine mobile receptors. Dynamic reorganization of actin structures occurs over seconds, making the location
Fluorometric assay for quantitation of biotin covalently attached to proteins and nucleic acids.
Journal:Biotechniques
PubMed ID:18019342
As a component of the (strept)avidin affinity system, biotin is often covalently linked to proteins or nucleic acids. We describe here a microplate-based high-throughput fluorometric assay for biotin linked to either proteins or nucleic acids based on fluorescence resonance energy transfer (FRET). This assay utilizes a complex of Alexa Fluoro
One at a time, live tracking of NGF axonal transport using quantum dots.
Journal:Proc Natl Acad Sci U S A
PubMed ID:17698956
Retrograde axonal transport of nerve growth factor (NGF) signals is critical for the survival, differentiation, and maintenance of peripheral sympathetic and sensory neurons and basal forebrain cholinergic neurons. However, the mechanisms by which the NGF signal is propagated from the axon terminal to the cell body are yet to be
Coupling of biotin-(poly(ethylene glycol))amine to poly(D,L-lactide-co-glycolide) nanoparticles for versatile surface modification.
Journal:Bioconjug Chem
PubMed ID:17590034
Generally, polymeric nanoparticles (NP) for drug targeting are designed to entrap the drug moiety in the core and to present the targeting moiety on the surface. However, in most cases, common preparation techniques of polymeric NP need to be specifically arranged for each compound to be entrapped or attached. In