Phusion Blood Direct PCR Kit
Phusion Blood Direct PCR Kit
Thermo Scientific™

Phusion Blood Direct PCR Kit

Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
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Catalog NumberNo. of Reactions
F547L500 Reactions
F547S100 Reactions
Catalog number F547L
Price (CNY)
3,782.00
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5,402.00
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No. of Reactions:
500 Reactions
Request bulk or custom format
Price (CNY)
3,782.00
飞享价
Ends: 31-Dec-2025
5,402.00
Save 1,620.00 (30%)
Each
Add to cart
Thermo Scientific Phusion Blood Direct PCR Kit is for use in the amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase exhibits high resistance to PCR inhibitors found in blood and retains polymerase activity even with samples containing 40% whole blood.

This DNA polymerase exhibits exceptional accuracy in proofreading and makes the Phusion Blood Direct PCR Kit one of the best choices for demanding applications, such as cloning. The Phusion Blood Direct PCR Kit is versatile and permits analysis of blood samples from various species and with different anticoagulants or storage conditions.

Features

  • No need for time-consuming and expensive DNA purification steps
  • Simple protocol requires minimal hands-on time
  • Extremely short PCR protocol times
  • Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product

Notes

  • The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Hot StartBuilt-In Hot Start
No. of Reactions500 Reactions
OverhangBlunt
PolymerasePhusion Blood II DNA Polymerase
Product TypeDirect PCR Kit
Quantity500 reactions
Reaction FormatSeparate Components
Sample TypeBlood
Shipping ConditionWet or Dry Ice
Starting MaterialDNA
Concentration2 U/μL
GC-Rich PCR PerformanceHigh
PCR MethodDirect PCR
Reaction SpeedFast
Unit SizeEach
Contents & Storage
• Phusion Blood II DNA Polymerase, 200 μL
• 2X Phusion Blood PCR Buffer with dNTPs and MgCl2, 5 x 1.25 mL
• Universal Control Primer Mix, 40 μL
• 50 mM EDTA, 0.5 mL
• 50 mM MgCl2, 1.5 mL
• DMSO, 0.5 mL

Store at -20°C.

Frequently asked questions (FAQs)

What is your recommendation to take a sample from plant or animal tissue for Direct PCR?

In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.

Can additional markers be added to Thermo Scientific Animal Genotyping kits?

No, extra markers cannot be added to any of the Animal Genotyping kits. All primer mixes are optimized to work in a multiplex PCR. Addition of one or more primers would require optimization of the primer concentrations and the PCR protocol.

Are primers from Thermo Scientific Animal Genotyping kits available separately for specific loci?

We do not provide primers for specific locii separately. They come only in a multiplex format. If one or several loci did not amplify from the sample, faint signals can be a result of low DNA amounts or impurities. Some loci may be more sensitive towards purity of DNA used and may vary from sample to sample. On rare occasions, an animal can have a deletion in the marker region.

Do Thermo Scientific Animal Genotyping kits include an allelic ladder?

No, none of the genotyping kits contain an allelic ladder. Animal STR loci are highly polymorphic. Each allele has too many variants to make a universal allelic ladder.

Why are Control primers provided in the Direct PCR Kits?

It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.

View all Phusion Blood Direct PCR Kit FAQs