ONE Broth Listeria Ready Bag

ONE Broth Listeria is a selective enrichment broth for Listeria species in food samples in 24 hours. Novel enrichment broth balances a mix of peptones, carbohydrates, and salts for optimal resuscitation, recovery, and growth of even low numbers of Listeria spp. from food samples in 24 hours as part of the Listeria Precis™ detection method.

Ready Bags are carefully designed to provide an effective and dependable way to enrich food samples for subsequent pathogen isolation, fully compliant with ISO standards. Key features and benefits include:

• Ready-to-Use: Our pre-prepared Ready Bags save time by eliminating the need for preparation
• Easy to Transport and Store: Equipped with ergonomic handles for simple movement and storage
• 1-Year Shelf Life
• Available in Packs of 3 bags
• Storage Conditions: 2°C to 12°C
• Tamper-evident ports on the Ready Bags help prevent contamination; they are also more durable and resistant to breaking
• Sturdy hanging apertures reduce bench space and improve dispensing efficiency

 
Testing Protocol

• Add 25 g of food sample to 225 mL of ONE Broth-Listeria and stomach for a minimum of 30 seconds to mix the sample
• Incubate the broth without agitation at 30°C for 24 hours
• Gently agitate the bag, then using a microbiological loop, remove 10 μL and inoculate onto a suitable agar medium e.g. Brilliance Listeria Agar (Cat. No. CM1080, SR0222, and SR0228) and incubate appropriately (37°C for 24-48 hours)
• Confirm presumptive colonies on the agar plates as Listeria monocytogenes or Listeria spp. by appropriate methods, e.g. Gram-stain, catalase, oxidase, Oxoid™ Listeria Latex Test Kit (Cat. No. DR1126), Oxoid™ O.B.I.S. mono (Cat. No. ID0600), Microbact Listeria 12L (Cat. No. MB1128)