Tissue Extraction Reagent I
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Tissue Extraction Reagent I
Invitrogen™

Tissue Extraction Reagent I

Tissue Extraction Reagent I is a ready-to-use Tris-based lysis buffer for total protein extraction from tissue samples by homogenization. CellRead more
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Catalog NumberQuantity
FNN0071100 ml
Catalog number FNN0071
Price (CNY)
1,535.00
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Ends: 31-Dec-2025
2,024.00
Save 489.00 (24%)
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Quantity:
100 ml
Price (CNY)
1,535.00
Online Exclusive
Ends: 31-Dec-2025
2,024.00
Save 489.00 (24%)
Each
Add to cart
Tissue Extraction Reagent I is a ready-to-use Tris-based lysis buffer for total protein extraction from tissue samples by homogenization. Cell lysates prepared with Tissue Extraction Reagent I are compatible with immunoassays such as ELISA, western blot, and Luminex assay.

Buffer formulation: 50 mM Tris, pH 7.4, 250 mM NaCl, 5 mM EDTA, 2 mM Na3VO4, 1 mM NaF, 20 mM Na4P2O7, 0.02% NaN3, detergent

This cell lysis buffer does not contain protease inhibitors and should be supplemented with 1 mM PMSF and protease inhibitor cocktail just prior to use.

Tissue Extraction Reagent I may be supplemented with salts, reducing agents, or chelating agents as needed.

View all available ELISA lysis buffers and reagents.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Luminex Assay, Western Blot, Sample preparation for ELISA
Product TypeTissue Extraction Reagent
Quantity100 ml
Shipping ConditionDry Ice
Label TypeEnzyme Labeled
Unit SizeEach
Contents & Storage
Buffer. Stable for 2-3 weeks at 2-8°C or for up to 1 year when apportioned into working aliquots and stored at ≤20°C.

Frequently asked questions (FAQs)

What extraction reagents are recommended for efficient mouse tissue analysis?

We have 5 different cell and tissue extraction buffers suitable for preparing mouse cell and tissue extracts. These buffers can be used to extract cells and tissues from many other species as well. The exact compositions of all of our buffers are proprietary, but they are similar to those described by many researchers.

Four of these buffers can be used to prepare extracts which can be analyzed with our ELISA and Luminex kits and by Western blotting. Our Cell Extraction Buffer (FNN0011) contains extra phosphatase inhibitors and resembles the RIPA formulation that many people use. Our Tissue Extraction Reagents I (FNN0071) and II (FNN0081) contain different concentrations of NaCl and different surfactants, but are otherwise similar to each other. For those who prefer using an extraction buffer containing the detergent NP-40, we have our NP-40 Lysis Buffer (FNN0021). Finally, we sell a Denaturing Cell Extraction buffer (FNN0091) which contains 3 detergents and a chaotropic agent. Extracts prepared with FNN0091 can be analyzed with our ELISA kits and by Western blotting only. These buffers do not contain protease inhibitors, which the investigator should add right before use.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the difference in application between Tissue Extraction Reagent I (Cat. No. FNN0071) and Tissue Extraction Reagent II (Cat. No. FNN0081)?

The main difference is that Tissue Extraction Reagent II is more stringent than Tissue Extraction Reagent I. Additionally, please note that Tissue Extraction Reagent I contains EDTA which may not be compatible with some downstream applications.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

Is Tissue Extraction Reagent I (Cat. No. FNN0071) compatible with the BCA Protein Assay Kit (Cat. No. 23227, 23225)?

Sorry, we have not tested the compatibility of Tissue Extraction Reagent I with our protein assays.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

During my ProcartaPlex assay data analysis, I am getting a warning message that there is high bead aggregation. What should I do?

Here are possible causes and solutions for this issue:

- Check the protocol settings (make sure you select the correct DD settings).
- Check the level of sheath fluid and empty the waste.
- Before acquiring the plate, run calibration and verification beads on the Luminex instrument.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

During my ProcartaPlex assay data analysis, the beads fall below or to the lower left of their bead region on the bead map. Why is this?

Here are possible causes and solutions for this issue:

This usually indicates that the beads have been photobleached. This problem can also be caused by exposing the beads to organic solvents. Unfortunately, the assay will have to be repeated because the beads cannot be restored. The beads must be protected from light and organic solvents.
Alternatively, the instrument may be off in its measurements or you may have a calibration issue. Call the manufacturer for a service appointment.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Tissue Extraction Reagent I FAQs