Hoechst 34580
Hoechst 34580
Citations & References (23)
Invitrogen™

Hoechst 34580

This bisbenzimide dye, Hoechst 34580, is related to the important minor-groove binding, dsDNA-selective dyes Hoechst 33258 and Hoechst 33342. HoechstRead more
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Catalog NumberQuantity
H214865 mg
Catalog number H21486
Price (CNY)
2,888.00
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Ends: 31-Dec-2025
3,837.00
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Quantity:
5 mg
Price (CNY)
2,888.00
Online Exclusive
Ends: 31-Dec-2025
3,837.00
Save 949.00 (25%)
Each
Add to cart
This bisbenzimide dye, Hoechst 34580, is related to the important minor-groove binding, dsDNA-selective dyes Hoechst 33258 and Hoechst 33342. Hoechst 34580 has somewhat longer-wavelength spectra than the other Hoechst dyes when bound to nucleic acids [Howard Shapiro, personal communication].

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeHoechst 34580
Excitation Wavelength Range392⁄440
For Use With (Equipment)Fluorescence Microscope
FormSolid
FormatTube(s)
Quantity5 mg
Shipping ConditionRoom Temperature
Solubilitywater, DMF or DMSO (Dimethylsulfoxide)
Product TypeNucleic Acid Stain
SubCellular LocalizationNucleic Acids, Nucleus
Unit SizeEach
Contents & Storage
Contains 1 vial of Hoechst 34580 (5 mg).
Store in freezer -25°C and protect from light.

Frequently asked questions (FAQs)

Is DAPI a good live-cell nuclear label?

DAPI is considered a semi-permeant/impermeant nucleic acid stain. Staining of nucleic is dependent upon the cell line in its performance. Some cell lines will label with DAPI, others not at all, and others label inconsistenly. Instead, we recommend using either Hoechst 33342 or Hoechst 33258, which have the same wavelength and binding mode as DAPI (at the A-T minor groove) but are readily cell-permeant.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label the nuclei of live cells and track them over time. Can I use DAPI for this?

We do not recommend doing this. DAPI is considered to be a semi-permeant/impermeant nucleic acid stain. DAPI staining of live cells may be inconsistent. It is best used as a counterstain for fixed samples. Other cell permeable nucleic acid stains, such as Hoechst or the SYTO dyes may affect cellular function.

For mammalian cells, we recommend using the CellLight Nucleus transduction reagents, available in CFP, GFP and RFP. With these reagents, the cells are transduced overnight in a single labeling step and the next day the nuclei will fluoresce. The label may be retained for 3-5 days and should not affect cell function. Cytoplasmic cell tracking dyes such as the CellTracker dyes may also be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?

DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (23)

Citations & References
Abstract
The inflammatory mediator leukotriene D4 induces beta-catenin signaling and its association with antiapoptotic Bcl-2 in intestinal epithelial cells.
Authors:Mezhybovska M, Wikström K, Ohd JF, Sjölander A
Journal:J Biol Chem
PubMed ID:16407243
'Increased levels of the inflammatory mediator leukotriene D4 (LTD4) are present at sites of inflammatory bowel disease, and such areas also exhibit an increased risk for subsequent cancer development. It is known that LTD4 affects the expression of many proteins that influence survival and proliferation of intestinal epithelial cells. We ... More
ATP-dependent interaction of the cytosolic domains of the inwardly rectifying K+ channel Kir6.2 revealed by fluorescence resonance energy transfer.
Authors:Tsuboi T, Lippiat JD, Ashcroft FM, Rutter GA
Journal:Proc Natl Acad Sci U S A
PubMed ID:14681552
'ATP-sensitive K(+) (K(ATP)) channels play important roles in the regulation of membrane excitability in many cell types. ATP inhibits channel activity by binding to a specific site formed by the N and C termini of the pore-forming subunit, Kir6.2, but the structural changes associated with this interaction remain unclear. Here, ... More
Nucleic acid binding agents exert local toxic effects on neurites via a non-nuclear mechanism.
Authors:Pin S, Chen H, Lein PJ, Wang MM
Journal:J Neurochem
PubMed ID:16441515
'The mechanism by which drugs that target nucleic acids cause neurotoxicity is not well described. We characterized the neurotoxicity of Hoechst 33342 (bis-benzimide), a common cell permeable nuclear dye, in primary neuronal cultures. The mechanism of cell death was not apoptotic, as death is rapid, not accompanied by typical nuclear ... More
Real-time multiple particle tracking of gene nanocarriers in complex biological environments.
Authors:Lai SK, Hanes J,
Journal:Methods Mol Biol
PubMed ID:18470640
'Complex biological fluids, such as the vast and molecularly crowded cell cytoplasm and the highly viscoelastic mucus that protects many entry ways to the body, pose significant barriers to efficient gene delivery. Understanding the dynamics of gene carriers in such environments allows insight that leads to rational improvements in gene ... More
Isolation, structure elucidation and cytotoxic evaluation of endiandrin B from the Australian rainforest plant Endiandra anthropophagorum.
Authors:Davis RA, Barnes EC, Longden J, Avery VM, Healy PC,
Journal:Bioorg Med Chem
PubMed ID:19138858
Chemical investigations of the DCM extract from the roots of Endiandra anthropophagorum resulted in the isolation of a new cyclobutane lignan endiandrin B (1), together with the known natural products, endiandrin A (2), and (-)-dihydroguaiaretic acid (3). The structure of 1 was determined by extensive spectroscopic analyses, and confirmed by ... More
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