HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit offers a complete set of reagents for performing image-based high-content screening (HCS) assays to characterize phospolipidosis and steatosis, toxic side effects on lipid metabolism that can be triggered by drugs or other compounds. The kit supplies LipidTOX™ Red phospholipid stain and LipidTOX™ Green neutral lipid stain, which can be used sequentially for the analysis of phospholipidosis and steatosis, respectively, or can be used in isolation for single-parameter analysis. Both fluorescent probes can be easily detected with fluorescence microscopes and can be quantified with stand-alone image analysis software or the built-in image analysis software of most HCS readers. In addition to the phospholipid and neutral lipid stains, this kit supplies the nuclear stain Hoechst 33342, propranolol and cyclosporin A (control compounds), and DMSO. Sufficient reagents are supplied for 2, 96-well plates (H34157) or 10, 96-well plates (H34158).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection Method
Fluorescent
For Use With (Equipment)
High Content Analysis Instrument
Product Type
Phospholipidosis and Steatosis Detection Kit
Dye Type
Other Label(s) or Dye(s)
Format
96-well plate
Product Line
LipidTOX™, Molecular Probes™
Shipping Condition
Room Temperature
Contents & Storage
When stored at -20°C, desiccated, and protected from light, the kit is stable for at least 6 months.