ImaGene Red™ C12RG lacZ Gene Expression Kit
ImaGene Red&trade; C<sub>12</sub>RG <i>lacZ</i> Gene Expression Kit
Citations & References (4)
Invitrogen™

ImaGene Red™ C12RG lacZ Gene Expression Kit

The ImaGene Red™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include aRead more
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Catalog NumberQuantity
I29061 kit
Catalog number I2906
Price (CNY)
10,200.00
Each
Add to cart
Quantity:
1 kit
Price (CNY)
10,200.00
Each
Add to cart
The ImaGene Red™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12RG. Once inside the cell, the substrates are cleaved by β-galactosidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane. In addition to the C12RG, the kits also include the broad-spectrum β-galactosidase inhibitor, PETG, and chloroquine diphosphate for inhibiting acidic hydrolysis of the substrate.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assayβ-Gal (lacZ) Assay
Detection MethodFluorescence
Product LineDetectaGene
Product Typeβ-Galactosidase Reporter Gene Assay System
Quantity1 kit
Shipping ConditionRoom Temperature
SubstrateC12RG
Substrate PropertiesChemical Substrate
Substrate TypeBeta-Gal Substrate
Target EnzymeBeta-Galactosidase
FormatKit
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.

Citations & References (4)

Citations & References
Abstract
Vital staining of cardiac myocytes during embryonic stem cell cardiogenesis in vitro.
Authors:Metzger JM, Lin WI, Samuelson LC,
Journal:Circ Res
PubMed ID:8635211
'Mouse embryonic stem (ES) cells differentiate in vitro into a variety of cell types, including spontaneously contracting cardiac myocytes. The primary aim of this work was to use vital stain techniques for real-time detection of developing cardiac myocytes in ES cell differentiation cultures. The -440 to +6 human cardiac alpha-actin ... More
Activity of Hb9 interneurons during fictive locomotion in mouse spinal cord.
Authors:Kwan AC, Dietz SB, Webb WW, Harris-Warrick RM,
Journal:J Neurosci
PubMed ID:19759307
'Hb9 interneurons (Hb9 INs) are putative components of the mouse spinal locomotor central pattern generator (CPG) and candidates for the rhythm-generating kernel. Studies in slices and hemisected spinal cords showed that Hb9 INs display TTX-resistant membrane potential oscillations, suggesting a role in rhythm generation. To further investigate the roles of ... More
Functional ephrin-B2 expression for promotive interaction between arterial and venous vessels in postnatal neovascularization.
Authors:Hayashi S, Asahara T, Masuda H, Isner JM, Losordo DW,
Journal:Circulation
PubMed ID:15851594
'Ephrin-B2, one of the transmembrane ligands, is a genetic marker of arterial endothelial cells (ECs) at embryonic stages and is essential for cardiovascular development, but its roles in ischemic cardiovascular disease are not well understood. In this study, we focused on the function of ephrin-B2 in postnatal neovascularization. We found ... More
Fluorescence-activated cell sorting (FACS) of Drosophila hemocytes reveals important functional similarities to mammalian leukocytes.
Authors:Tirouvanziam R, Davidson CJ, Lipsick JS, Herzenberg LA
Journal:Proc Natl Acad Sci U S A
PubMed ID:14976247
Drosophila is a powerful model for molecular studies of hematopoiesis and innate immunity. However, its use for functional cellular studies remains hampered by the lack of single-cell assays for hemocytes (blood cells). Here we introduce a generic method combining fluorescence-activated cell sorting and nonantibody probes that enables the selective gating ... More