Image-iT™ TMRM Reagent (mitochondrial membrane potential indicator)
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Image-iT™ TMRM Reagent (mitochondrial membrane potential indicator)
Citations & References (5)
Invitrogen™

Image-iT™ TMRM Reagent (mitochondrial membrane potential indicator)

Healthy mitochondrial membranes maintain a difference in electrical potential between the interior and exterior of the organelle, referred to asRead more
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Catalog NumberQuantity
I343615 x 100 μL
Catalog number I34361
Price (CNY)
2,010.00
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Ends: 31-Dec-2025
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Quantity:
5 x 100 μL
Price (CNY)
2,010.00
飞享价
Ends: 31-Dec-2025
2,723.00
Save 713.00 (26%)
Each
Add to cart
Healthy mitochondrial membranes maintain a difference in electrical potential between the interior and exterior of the organelle, referred to as a membrane potential. Tetramethylrhodamine, methyl ester (TMRM) is a cell-permeant dye that accumulates in active mitochondria with intact membrane potentials. If the cells are healthy and have functioning mitochondria, the signal will be bright. Upon loss of the mitochondrial membrane potential, TMRM accumulation will cease and the signal will dim or disappear. TMRM signal can be detected with fluorescence microscopy, flow cytometry, cell sorting, high-throughput screening, and high content analysis.

• For live-cell dynamic study of mitochondrial membrane potential (not fixable)
• Detectable with RFP/TRITC filter set (absorbance peak: 548 nm, emission peak: 574 nm)
• Mutiplexable with green, deep red, violet, and blue emission dyes, such as GFP, DAPI, Hoechst, HCS LipidTOX, and LysoTracker Deep Red

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity5 x 100 μL
Shipping ConditionWet Ice
ColorOrange
Product LineMolecular Probes
Product TypeReagent
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

For a final staining solution, we can add TMRM directly into cell growth medium. Can this media contain serum?

Yes, you may add TMRM to media with serum. Serum components may bind dye non-specifically so you may need a higher final working concentration for staining samples in media containing serum relative to staining the same cells in a non-serum media or buffer.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is TMRM different from the MitoTracker dyes?

TMRM is a dynamic mitochondrial stain. There is no covalent attachment of TMRM to the mitochondria and because of this, it can move in and out of the mitochondria dependent upon changes in the membrane potential. The MitoTracker dyes on the other hand possess a reactive chloromethyl group that allows for their covalent attachment to mitochondria. As a result, once they accumulate within mitochondria that have a normal membrane potential, they are retained.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is included in the Image-iT TMRM Reagent (Cat No. I34361)?

The Image-iT TMRM Reagent is a ready-to-use 1,000X concentrated stock solution of the TMRM dye. It contains 5 vials of 100 µM of TMRM in neat DMSO, with 100 µL volume in each vial. TMRM powder (Cat No. T668) is not included in this kit.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (5)

Citations & References
Abstract
miR-450a Acts as a Tumor Suppressor in Ovarian Cancer by Regulating Energy Metabolism.
Authors:Muys BR, Sousa JF, Plaça JR, de Araújo LF, Sarshad AA, Anastasakis DG, Wang X, Li XL, de Molfetta GA, Ramão A, Lal A, Vidal DO, Hafner M, Silva WA
Journal:Cancer Res
PubMed ID:31101765
'Dysregulation of miRNA expression is associated with multiple diseases, including cancers, in which small RNAs can have either oncogenic or tumor suppressive functions. Here we investigated the potential tumor suppressive function of miR-450a, one of the most significantly downregulated miRNAs in ovarian cancer. RNA-seq analysis of the ovarian cancer cell ... More
Ursodeoxycholic acid protects against cisplatin-induced acute kidney injury and mitochondrial dysfunction through acting on ALDH1L2.
Authors:Yang Y, Liu S, Gao H, Wang P, Zhang Y, Zhang A, Jia Z, Huang S
Journal:Free Radic Biol Med
PubMed ID:32004633
'Mitochondrial dysfunction plays an important role in acute kidney injury (AKI). Thus, the agents improving the mitochondrial function could be beneficial for treating AKI. Ursodeoxycholic acid (UDCA) has been demonstrated to prevent mitochondrial dysfunction under pathology, however, its role in AKI and the underlying mechanism remain unknown. This study aimed ... More
Targeting mitochondria-associated membranes as a potential therapy against endothelial injury induced by hypoxia.
Authors:Yang YD, Li MM, Xu G, Zhang EL, Chen J, Sun B, Chen DW, Gao YQ
Journal:J Cell Biochem
PubMed ID:31241212
Mitochondrial dysfunction plays a principal role in hypoxia-induced endothelial injury, which is involved in hypoxic pulmonary hypertension and ischemic cardiovascular diseases. Recent studies have identified mitochondria-associated membranes (MAMs) that modulate mitochondrial function under a variety of pathophysiological conditions such as high-fat diet-mediated insulin resistance, hypoxia reoxygenation-induced myocardial death, and hypoxia-evoked vascular smooth ... More
Barrier-to-Autointegration Factor 1 Protects against a Basal cGAS-STING Response.
Authors:Ma H, Qian W, Bambouskova M, Collins PL, Porter SI, Byrum AK, Zhang R, Artyomov M, Oltz EM, Mosammaparast N, Miner JJ, Diamond MS
Journal:mBio
PubMed ID:32156810
Although the pathogen recognition receptor pathways that activate cell-intrinsic antiviral responses are well delineated, less is known about how the host regulates this response to prevent sustained signaling and possible immune-mediated damage. Using a genome-wide CRISPR-Cas9 screening approach to identify host factors that modulate interferon-stimulated gene (ISG) expression, we identified ... More
Mitochondrial Pyruvate Carrier 1 Promotes Peripheral T Cell Homeostasis through Metabolic Regulation of Thymic Development.
Authors:Ramstead AG, Wallace JA, Lee SH, Bauer KM, Tang WW, Ekiz HA, Lane TE, Cluntun AA, Bettini ML, Round JL, Rutter J, O'Connell RM
Journal:Cell Rep
PubMed ID:32130894
Metabolic pathways regulate T cell development and function, but many remain understudied. Recently, the mitochondrial pyruvate carrier (MPC) was identified as the transporter that mediates pyruvate entry into mitochondria, promoting pyruvate oxidation. Here we find that deleting Mpc1, an obligate MPC subunit, in the hematopoietic system results in a specific reduction ... More