Acetate Buffer (1 M, pH 5.5)
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Acetate Buffer (1 M, pH 5.5)
Acetate Buffer (1 M, pH 5.5)
Acetate Buffer (1 M, pH 5.5)
Thermo Scientific Chemicals

Acetate Buffer (1 M, pH 5.5)

CAS: 127-09-3 | C2H3NaO2 | 82.03 g/mol
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Catalog number J61033.AP
also known as J61033-AP
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500mL
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Chemical Identifiers
CAS127-09-3
IUPAC Namesodium acetate
Molecular FormulaC2H3NaO2
InChI KeyVMHLLURERBWHNL-UHFFFAOYSA-M
SMILES[Na+].CC([O-])=O
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SpecificationsSpecification SheetSpecification Sheet
Appearance (Color)Clear colorless
FormLiquid
Concentration1M
MethodFiltered through 0.22-micron filter
pH5.30-5.70
Acetate, 1M buffer soln., pH, 5.5 it is been widely used in for precipitation of nucleic acids and preparation of gel stains for protein gel electrophoresis. Sodium acetate buffer solution is suitable for precipitation of DNA, elution of proteins and for use in molecular biology applications such as ChIP-on-ChIP assay and in vitro enzyme inhibition assay.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Acetate, 1M buffer soln., pH, 5.5 it is been widely used in for precipitation of nucleic acids and preparation of gel stains for protein gel electrophoresis. Sodium acetate buffer solution is suitable for precipitation of DNA, elution of proteins and for use in molecular biology applications such as ChIP-on-ChIP assay and in vitro enzyme inhibition assay.

Solubility
Soluble in water (246 g/L) at 20°C, and ethanol.

Notes
Contains sodium acetate and glacial acetic acid, pH 5.5
RUO – Research Use Only

General References:

  1. Tong Ihn Lee et. al. Chromatin immunoprecipitation and microarray-based analysis of protein location. Nature Protocols. 2006, 1,(2), 729-748.
  2. Jaspreet Kaur et. al. In silico screening, structure-activity relationship, and biologic evaluation of selective pteridine reductase inhibitors targeting visceral leishmaniasis. Antimicrobial Agents and Chemotherapy. 2011, 55,(2), 659-666.