HEPES-buffered saline, pH 6.5 (5X)
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HEPES-buffered saline, pH 6.5 (5X)
HEPES-buffered saline, pH 6.5 (5X)
HEPES-buffered saline, pH 6.5 (5X)
Thermo Scientific Chemicals

HEPES-buffered saline, pH 6.5 (5X)

CAS: 7365-45-9 | C8H18N2O4S | 238.30 g/mol
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500 mL
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Catalog number J61239.AP
also known as J61239-AP
Price (CNY)
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Quantity:
500 mL
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Chemical Identifiers
CAS7365-45-9
IUPAC Name2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
Molecular FormulaC8H18N2O4S
InChI KeyJKMHFZQWWAIEOD-UHFFFAOYSA-N
SMILESOCCN1CCN(CCS(O)(=O)=O)CC1
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SpecificationsSpecification SheetSpecification Sheet
pH6.35-6.65
ConcentrationHEPES: 125 mM
ConcentrationSodium Chloride: 750 mM
HEPES is typically used in cell culture at concentration between 5mM to 30 mM. It is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
HEPES is typically used in cell culture at concentration between 5mM to 30 mM. It is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.

Solubility
Fully miscible in water.

Notes
Contains 125mM HEPES and 750mM sodium chloride, pH 6.5
RUO – Research Use Only

General References:

  1. Sheila Timmons; Jack Hawiger. Separation of human platelets from plasma proteins including factor VIIIVWF by a combined albumin gradient-gel filtration method using hepes buffer. Thrombosis Research. 1978, 12,(2), 297-306.
  2. Raymond Wai-Yin Sun; Rong Chen; Nancy P.-Y. Chung; Chi-Ming Ho; Chen-Lung Steve Lin; Chi-Ming Che. Silver nanoparticles fabricated in Hepes buffer exhibit cytoprotective activities toward HIV-1 infected cells. Chem. Commun. 2005, (40), 5059-5061.