LDS-sample buffer (4X), non-reducing

Name: LDS-sample buffer (4X), non-reducing
SKU: J61894.AC

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Quantity:

25 mL

50 mL

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Catalog number J61894.AC

also known as J61894-AC

Price (CNY)

Quantity:

25 mL

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Specifications

ColorBlue

Recommended StorageAmbient temperatures

Concentration4X

Physical FormLiquid

Quantity25 mL

LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

Solubility
Not miscible in water.

Notes
This buffer contains: 988mM Tris-HCl, 2.04mM EDTA, 8% LDS, 40% glycerol, 0.88% Coomassie blue and 0.7mM phenol red at pH 8.5.

RUO – Research Use Only

General References:

Yi Wang; Qiaozhen Lu; Shiaw-Lin Wu; Barry L. Karger and William S. Hancock. Characterization and Comparison of Disulfide Linkages and Scrambling Patterns in Therapeutic Monoclonal Antibodies: Using LC-MS with Electron Transfer Dissociation. Anal. Chem. 2011, 83, (8), 3133-3140.
Dan Lu; Zhenping Zhu. Construction and Production of an IgG-Like Tetravalent Bispecific Antibody for Enhanced Therapeutic Efficacy. Therapeutic Antibodies. 2008, 525, 377-404.

Frequently asked questions (FAQs)

At what temperature should I store LDS-sample buffer (4X), non-reducing (Cat. No. J61894.xx)?

LDS-sample buffer (4X), non-reducing should be stored at room temperature (20-25 degrees Celsius) upon receipt.

What protocol should I use for LDS-sample buffer (4X), non-reducing (Cat. No. J61894.xx)?

We recommend the following protocol for use of LDS-sample buffer (4X), non-reducing:
1. Mix one part LDS sample buffer to three parts protein (e.g., 5 µL sample buffer + 15 µL protein sample). Failure to properly dilute may result in a diffuse dye front.
2. Heat mixture at 100°C for 3-5 minutes.
3. Allow sample to cool back to room temperature.
4. Load sample onto SDS-polyacrylamide gel and begin electrophoresis.
5. Stop electrophoresis when the dye front nears the bottom of the gel.