Trichloroacetic acid, 5% w/v aq. soln.
Trichloroacetic acid, 5% w/v aq. soln.
Trichloroacetic acid, 5% w/v aq. soln.
Thermo Scientific Chemicals

Trichloroacetic acid, 5% w/v aq. soln.

Protein precipitation reagent | CAS: 76-03-9 | C2HCl3O2 | 163.378 g/mol
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Quantity:
500 mL
1 L
Catalog number J62366.AP
also known as J62366-AP
Price (CNY)
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Quantity:
500 mL
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Chemical Identifiers
CAS76-03-9
IUPAC Nametrichloroacetic acid
Molecular FormulaC2HCl3O2
InChI KeyYNJBWRMUSHSURL-UHFFFAOYSA-N
SMILESOC(=O)C(Cl)(Cl)Cl
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SpecificationsSpecification SheetSpecification Sheet
Appearance (Color)Clear colorless
FormLiquid
Assay from Supplier's CofA4.75-5.25%
Trichloroacetic acid, is traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. It is also used as a decalcifier and fixative in microscopy in protein sequencing, detecting albumin and organic synthesis. A protocol for the precipitation of nucleic acids can be found in Molecular Cloning.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Trichloroacetic acid, is traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. It is also used as a decalcifier and fixative in microscopy in protein sequencing, detecting albumin and organic synthesis. A protocol for the precipitation of nucleic acids can be found in Molecular Cloning.

Solubility
Soluble in water (1200 mg/ml at 20°C), alcohol, ether, ethanol (very soluble), and carbon tetrachloride.

Notes
Store under nitrogen. Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Keep away from strong oxidizing agents, strong bases, smines.
RUO – Research Use Only

General References:

  1. RW Blakesley.; JA Boezi. A new staining technique for proteins in polyacrylamide gels using Coomassie Brilliant Blue G250. Analytical biochemistry. 1977, 82(2), 580-582.
  2. WH Marsh.; B Fingerhut.; H Miller. Automated and manual direct methods for the determination of blood urea. Clinical chemistry. 1965, 11(6), 624-627.