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Thermo Scientific Chemicals
Brilliant Blue R
CAS: 6104-59-2 | C45H44N3NaO7S2 | 825.97 g/mol
| Catalog Number | Quantity |
|---|---|
| J64297.18 also known as J64297-18 | 50 g |
Catalog number J64297.18
also known as J64297-18
Price (CNY)
-
Quantity:
50 g
Chemical Identifiers
CAS6104-59-2
IUPAC Namesodium {4-[(4-ethoxyphenyl)amino]phenyl}bis(4-{ethyl[(3-sulfonatophenyl)methyl]amino}phenyl)methylium
Molecular FormulaC45H44N3NaO7S2
InChI KeyHAEGGLCXLFWTBE-UHFFFAOYSA-M
SMILES[Na+].CCOC1=CC=C(NC2=CC=C(C=C2)[C+](C2=CC=C(C=C2)N(CC)CC2=CC=CC(=C2)S([O-])(=O)=O)C2=CC=C(C=C2)N(CC)CC2=CC=CC(=C2)S([O-])(=O)=O)C=C1
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Specifications
Specification Sheet
Specification SheetFormPowder
CommentSolubility: 1% in water, Complete dissolution, deep blue-black solution
Assay from Supplier's CofADye content: ≥75.0%
Appearance (Color)Dark purple to purple-brown
Brilliant Blue R is used for detection of protein bands following electrophoresis. It is a sensitive stain for protein detection in polyacrylamide gels, typically giving blue bands on a clear background with a sensitivity of 50-100 ng/band. It may be combined with other stains, such as silver stain, to distinguish different types of proteins. It displays metachromasia by appearing pink-red, rather than blue, when binding certain proteins, such as collagen and histone H1.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Brilliant Blue R is used for detection of protein bands following electrophoresis. It is a sensitive stain for protein detection in polyacrylamide gels, typically giving blue bands on a clear background with a sensitivity of 50-100
Brilliant Blue R is used for detection of protein bands following electrophoresis. It is a sensitive stain for protein detection in polyacrylamide gels, typically giving blue bands on a clear background with a sensitivity of 50-100
RUO – Research Use Only
General References:
- James Dzandu; M.E.Deh; D.L.Barratt; G.E.Wise. Detection of erythrocyte membrane proteins, sialoglycoproteins, and lipids in the same poly-acrylamide gel using a double-staining technique. Proceedings of the National Academy of Sciences. 1984, 81,(6), 1733-1737.
- H.Chen; H.Cheng; M.Bjerknes. One-step Coomassie brilliant blue R-250 staining of proteins in polyacrylamide gel. Analytical Biochemistry. 1993, 212,(1), 295-296.