Agarose, low EEO

Name: Agarose, low EEO
SKU: J66369.22

CAS: 9012-36-6

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Catalog number J66369.22

also known as J66369-22

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100 g

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Chemical Identifiers

CAS9012-36-6

IUPAC Name(2S,3R,4S,5R,6R)-2-{[(1S,3S,4S,5S,8R)-3-{[(2S,3R,4S,5S,6R)-2-{[(1S,3R,4S,5S,8R)-3,4-dihydroxy-2,6-dioxabicyclo[3.2.1]octan-8-yl]oxy}-3,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy}-4-hydroxy-2,6-dioxabicyclo[3.2.1]octan-8-yl]oxy}-6-(hydroxymethyl)oxane-3,4,5-triol

Molecular FormulaC24H38O19

InChI KeyMJQHZNBUODTQTK-WKGBVCLCSA-N

SMILESOC[C@H]1O[C@@H](O[C@@H]2[C@@H]3CO[C@H]2[C@H](O)[C@H](O[C@H]2[C@@H](O)[C@@H](CO)O[C@@H](O[C@@H]4[C@@H]5CO[C@H]4[C@H](O)[C@H](O)O5)[C@@H]2O)O3)[C@H](O)[C@@H](O)[C@H]1O

Specifications

Specification Sheet

Assay (special)EEO: 0.09 to 0.13

Appearance (Color)White to cream

Assay (special)Gel strength (1%): ≥1200 gm/cm2

FormPowder

DNaseNone detected

Agarose is useful in separation of nucleic acids electrophoretically, Suitable for isoelectric focusing, protein blotting and antibody separation Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Agarose is useful in separation of nucleic acids electrophoretically, Suitable for isoelectric focusing, protein blotting and antibody separation Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions.

Solubility
Soluble in water

Notes
Gel strength: >2400g/cm² (1.5% w/v); Gelling temperature: 35-37°C; EEO: 0.07-0.1

RUO – Research Use Only

General References:

James M. Bailey.; Norman Davidson. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Analytical Biochemistry. 1976, 70, (1), 75-85.
Carl-Bertil Laurell. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Analytical Biochemistry. 1966, 15, (1), 45-52.