Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial
Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial
Thermo Scientific™

Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

**********************Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at aRead more
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Catalog NumberNo. of Reactions
K0251200 reactions
K02534,000 Reactions
K02521,000 Reactions
Catalog number K0251
Price (CNY)
2,297.00
Each
Add to cart
No. of Reactions:
200 reactions
Price (CNY)
2,297.00
Each
Add to cart
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Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

Highlights

SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity—detects low copy number targets
Wide linear range—accurate quantification across 9 orders of magnitude
Reproducibility and convenience—ready-to-use 2x master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Maxima SYBR Green qPCR Master Mix, ROX Solution provided (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer. Supplied with nuclease-free water and a separate ROX vial.

Related Products
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
FormatTube
No. of Reactions200 reactions
PolymeraseTaq DNA Polymerase
Product LineMaxima
Product TypeReal Time PCR SYBR Master Mix
Quantity200 reactions
Sample TypeDNA, dsDNA
Sufficient For200 Reactions of 25 μL
Volume2 x 1.25 mL
Concentration2X
Detection MethodSYBR
For Use With (Application)Gene Expression
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
Contains:
• 2 x 1.25 mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 200 x 25 μL reactions.
• 50 μL ROX solution, 50 μM
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.