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Thermo Scientific™
GeneJET Plant RNA Purification Kit
GeneJET Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from wide variety of plant species and tissue types.
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| Catalog Number | Quantity |
|---|---|
| K0802 | 250 Preps |
| K0801 | 50 Preps |
Catalog number K0802
Price (CNY)
-
Quantity:
250 Preps
Thermo Scientific GeneJET Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from wide variety of plant species and tissue types.
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis. Purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis. RNA yields vary between different species, tissues, and age of tissue sample. See Table 1 for typical total RNA yields from various sources.
Highlights
- Fast—procedure completed in 20 min following cell lysis
- Convinient—protocol does not include clogging-prone lysate filtration step
- High yield—up to 65 μg of high purity and high integrity RNA from 50 mg plant sample
- Compatible with wide variety of samples (leaves, roots, sprouts and other parts of various plants)
Applications
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
- RT-PCR
- RT-qPCR
- Northern blotting
- Nuclease protection assay
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elution Volume50 μL
Final Product TypeTotal RNA
For Use With (Application)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Purification Time20 min. (After sample homogenization)
Quantity250 Preps
ScaleMini
Shipping ConditionRoom Temperature
Starting Material Amount≤100 mg
YieldUp to 65 μg
Isolation TechnologySilica Spin Column
Sample TypePlants
Unit SizeEach
Contents & Storage
• 200 mL Plant RNA Lysis Solution; store at room temperature
• 200 mL Wash Buffer WB 1 (concentrated); room temperature
• 2 x 100 mL Wash Buffer 2 (concentrated); room temperature
• 125 mL Water, Nuclease-free; room temperature
• 250 GeneJET RNA Spin Columns with Collection Tubes; For better long-term performance, it is recommended to store the purification columns at 2°C-8°C.
• 250 Collection Tubes (2 mL); room temperature
• 250 Collection Tubes (1.5 mL); room temperature
• 200 mL Wash Buffer WB 1 (concentrated); room temperature
• 2 x 100 mL Wash Buffer 2 (concentrated); room temperature
• 125 mL Water, Nuclease-free; room temperature
• 250 GeneJET RNA Spin Columns with Collection Tubes; For better long-term performance, it is recommended to store the purification columns at 2°C-8°C.
• 250 Collection Tubes (2 mL); room temperature
• 250 Collection Tubes (1.5 mL); room temperature