CCF2-AM
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CCF2-AM

CCF2-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF2 substrate which allows it to readily enter cells.Read more
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Catalog NumberQuantity
K10235 mg
Catalog number K1023
Price (CNY)
283,677.00
Each
Add to cart
Quantity:
5 mg
Price (CNY)
283,677.00
Each
Add to cart
CCF2-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF2 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF2-AM into its negatively charged form, CCF2 or CCF4, which is retained in the cytosol. CCF2 is a Fluorescence Resonance Energy Transfer (FRET) substrate which consists of a cephalosporin core linking 7-hydroxycoumarin to fluorescein. This product is to be used as a FRET-based substrate for β-lactamase as a sensitive reporter of mammalian gene expression in the GeneBLAzer® technology.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityCell-Permeant
For Use With (Equipment)Flow Cytometer, Microplate Reader
Product LineGeneBLAzer™
Quantity5 mg
Shipping ConditionRoom Temperature
SubstrateCCF2-AM
Detection MethodFluorescence
Substrate PropertiesChemical Substrate
Substrate TypeBeta-Lactamase Substrate
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C).

Frequently asked questions (FAQs)

Do you offer an alternative to GeneBLAzer In Vivo Detection Kit (Cat. No. 12578134)?

The following are two alternatives to the GeneBLAzer In Vivo Detection Kit:
- LiveBLAzer FRET-B/G Loading Kit with CCF2-AM (Cat. No. K1032, K1025)
- LiveBLAzer FRET-B/G Loading Kit with CCF4-AM (Cat. No. K1095, K1096, K1030)

Concerning CCF2-AM and CCF4-AM, these molecules differ by two carbons in the bridge that links the coumarin moiety to the lactam ring. Both are in the membrane-permeable, esterified forms, and can be used for assays in intact cells. CCF4-AM has better solubility properties than CCF2-AM and is thus best suited for screening applications. In addition, CCF4-AM has slightly better FRET and thus slightly lower background than CCF2-AM.

Note: We also offer the loading solutions as a separate kit, Loading Solutions (Cat. No. K1026), and CCF2-AM is also available as a standalone product (Cat. No. K1023).