β-Gal Staining Kit

Citations & References (3)

Invitrogen™

β-Gal Staining Kit

The β-Gal Staining Kit allows you to determine the percentage of transfected cells expressing lacZ. lacZ is a bacterial geneRead more

Catalog Number	Quantity
K146501	1 Kit

Catalog number K146501

Price (CNY)

6,941.00

Add to cart

Quantity:

1 Kit

Price (CNY)

6,941.00

Add to cart

The β-Gal Staining Kit allows you to determine the percentage of transfected cells expressing lacZ. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments. The gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates and its activity is easily assayed. β-galactosidase catalyzes the hydrolysis of X-gal, producing a blue color that can be easily visualized under a microscope.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assayβ-Gal (lacZ) Assay

Product TypeBeta-Gal Staining Kit

Quantity1 Kit

SubstrateX-Gal

TargetBeta-Galactosidase

Detection MethodColorimetric

Unit SizeEach

Contents & Storage

The β-Gal Staining Kit contains sufficient reagents to stain fifty 60 mm plates. The kit includes 10X PBS (phosphate buffered saline), X-gal, staining solutions A, B, and C, 10X fixative solution, and 10 μg of pcDNA™ 3.1/His/lacZ control vector. Store the 10X PBS at room temperature. All other components should be stored at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Citations & References (3)

Citations & References

Abstract

Use of enhanced green fluorescent protein to optimize and quantitate infection of target cells with recombinant retroviruses.

Authors:Cashion LM, Bare LA, Harvey S, Trinh Q, Zhu Y, Devlin JJ

Journal:Biotechniques

PubMed ID:10337486

'Recombinant retroviral vectors are useful tools for gene transfer in both gene therapy and research applications. An enhanced form of green fluorescent protein has been incorporated into recombinant retroviruses as a marker to follow infected cells. In this paper, we extended the use of the fluorescent reporter to quantify protein ... More

Endothelial induction of fgl2 contributes to thrombosis during acute vascular xenograft rejection.

Authors:Ghanekar A, Mendicino M, Liu H, He W, Liu M, Zhong R, Phillips MJ, Levy GA, Grant DR,

Journal:J Immunol

PubMed ID:15100314

Thrombosis is a prominent feature of acute vascular rejection (AVR), the current barrier to survival of pig-to-primate xenografts. Fibrinogen-like protein 2 (fgl2/fibroleukin) is an inducible prothrombinase that plays an important role in the pathogenesis of fibrin deposition during viral hepatitis and cytokine-induced fetal loss. We hypothesized that induction of fgl2 ... More

Phosphorylation of serine 256 is required for cAMP-dependent regulatory exocytosis of the aquaporin-2 water channel.

Authors:Fushimi K, Sasaki S, Marumo F

Journal:J Biol Chem

PubMed ID:9169447

The aquaporin-2 (AQP2) vasopressin water channel is translocated to the apical membrane upon vasopressin stimulation. Phosphorylation of serine 256 of AQP2 by cAMP-dependent protein kinase has been shown, but its relation to vasopressin-regulated translocation has not been elucidated. To address this question, wild type (WT) AQP2 and a mutant with ... More