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Invitrogen™
mRNA Catcher™ PLUS Purification Kit
The mRNA Catcher PLUS Purification Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format.
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| Catalog Number | Quantity | Product Type |
|---|---|---|
| K157002 | 96 Preps | Purification Kit |
| K157003 | 960 Preps | Purification Kit |
| K157004 also known as K1570-04 | 960 Preps | Buffers Only |
Catalog number K157002
Price (CNY)
6,182.00
Each
Quantity:
96 Preps
Product Type:
Purification Kit
Price (CNY)
6,182.00
Each
The mRNA Catcher PLUS Purification Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of locked nucleic acid (LNA) technology, the mRNA Catcher PLUS kit provides improved performance and a faster protocol, while preserving mRNA yield and purity.
The mRNA Catcher PLUS Purification Kit offers:
- Robust performance—high binding capacity per well (>80 ng/well)
- Reproducible results—low CVs from well-to-well and plate-to-plate (∼2%)
- Optimized protocols—for small sample sizes (100 cells, 4 mg tissue, and 40 μL blood)
- Compatible plate design—may be used with most PCR cyclers
How it works
mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or reversed-transcribed to cDNA directly in the well. The mRNA Catcher PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamLined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elution Volume80 μL
Final Product TypemRNA
For Use With (Application)RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
For Use With (Equipment)Robotic Liquid Handling Systems
High-throughput CompatibilityHigh-throughput Compatible
Product TypePurification Kit
Purification Time1.5 hr. (Including sample homogenization)
Quantity96 Preps
Shipping ConditionRoom Temperature
Starting Material AmountCells: 100–106, Tissue: 4–40 mg, Total RNA: 0.1–100 μg, Whole Blood: 40 μL
Yield>80 ng
Isolation TechnologyOligo dT Affinity
Sample TypeCells, Tissue, Total RNA, Whole Blood
Unit SizeEach
Contents & Storage
• 1 x 96-well mRNA Catcher™ PLUS Plate; store at 4°C
• 4 mL 2X Lysis Buffer L20; room temperature
• 30 mL Wash Buffer W15; room temperature
• 4 mL Dilution Buffer DL1; room temperature
• 8 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature
• 100 μL 0.5M DTT; 4°C
• 4 mL 2X Lysis Buffer L20; room temperature
• 30 mL Wash Buffer W15; room temperature
• 4 mL Dilution Buffer DL1; room temperature
• 8 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature
• 100 μL 0.5M DTT; 4°C