S.c. EasyComp™ Transformation Kit

Citations & References (3)

Invitrogen™

S.c. EasyComp™ Transformation Kit

The S.c. EasyComp™ Transformation Kit is designed for rapid preparation of transformation-competent Saccharomyces cerevisiae cells. Advantages over commonly used transformationRead more

Catalog Number	Quantity
K505001	1 kit

Catalog number K505001

Price (CNY)

7,297.00

Add to cart

Quantity:

1 kit

Price (CNY)

7,297.00

Add to cart

The S.c. EasyComp™ Transformation Kit is designed for rapid preparation of transformation-competent Saccharomyces cerevisiae cells. Advantages over commonly used transformation procedures such as spheroplast formation or LiCl methods include:

• Cells can be made competent in less than 30 minutes
• Competent cell preparation and transformation solutions are optimized for use with S. cerevisiae
are provided ready-to-use and tested to ensure successful yeast transformation
• Competent cells can be frozen for future use

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product TypeTransformation Kit

Quantity1 kit

Target Organism ClassS. cerevisiae

Product LineEasyComp

Unit SizeEach

Contents & Storage

The S.c. EasyComp™ Transformation Kit provides enough reagents to prepare 6 batches of competent cells. Each batch is sufficient for 20 transformations. The kit should be stored at +4°C. All components are guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

What are the different kinds of media used for culturing Pichia pastoris and S. cerevisiae?

Following are the rich and minimal media used for culturing Pichia pastoris and S. cerevisiae:

Rich Media:
S. cerevisiae and Pichia pastoris
YPD (YEPD): yeast extract, peptone, and dextrose
YPDS: yeast extract, peptone, dextrose, and sorbitol

Pichia pastoris only
BMGY: buffered glycerol-complex medium
BMMY: buffered methanol-complex medium

Minimal Media (also known as drop-out media):
S. cerevisiae
SC (SD): Synthetic complete (YNB, dextrose (or raffinose or galactose), and amino acids)

Pichia pastoris
MGY: minimal glycerol medium
MD: minimal dextrose
MM: minimal methanol
BMGH: buffered minimal glycerol
BMMH: buffered minimal methanol

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Will the Saccharomyces cerevisiae alpha-factor secretion signal be recognized by Schizosaccharomyces pombe?

S. pombe cannot generate P factor when P factor is replaced for alpha in the alpha factor gene. It can, however, produce alpha factor when alpha is replaced for P in the P factor gene. This is negative evidence that S. pombe can process its own mating factor cleavage sites, but not all the cleavage sites of the S. cerevisiae alpha factor. It is better to use a more generic signal sequence (rather than a pre- pro- signal sequence such as alpha). If it is necessary to go the pre- pro- route, it is better to use the S. pombe P factor leader rather than the S. cerevisiae alpha leader.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

For galactose induction of expression in S. cerevisiae, can I include additional carbon sources in the media to increase yeast growth without repressing expression from the GAL promoter?

Some researchers choose to grow yeast in medium containing 2% galactose as the sole carbon source during induction. However, yeast typically grow more quickly in induction medium containing 2% galactose plus 2% raffinose. Raffinose is a good carbon source for yeast, and unlike glucose, does not repress transcription from the GAL promoter. Raffinose is a trisaccharide of galactose, glucose, and fructose linked in that order. Most yeast can cleave the glucose-fructose bond, but not the galactose-glucose bond. Fructose is then used as a carbon source.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Which S. cerevisiae yeast strain do your kits contain?

We offer the INVSc1 yeast strain. It is a diploid strain for expression purposes only. It does not sporulate well and is therefore not suited for yeast genetic studies. The genotype and phenotype of the INVSc1 strain are as follows:

Genotype: MATa his3D1 leu2 trp1-289 ura3-52/MATalpha his3D1 leu2 trp1-289 ura3-52
Phenotype: His-, Leu-, Trp-, Ura-
Note that INVSc1 is auxotrophic for histidine, leucine, tryptophan, and uracil. The strain will not grow in SC minimal medium that is deficient in histidine, leucine, tryptophan, and uracil.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Can old premixed lithium acetate buffers be used for preparing and transforming Saccharomyces cerevisiae?

Stock buffers of TE, lithium acetate, and PEG can be stored. However, the combined solution used to prepare the cells for transformation must be made fresh every time. There is a loss in transformation efficiency if the solutions are not freshly prepared.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all S.c. EasyComp™ Transformation Kit FAQs

Citations & References (3)

Citations & References

Abstract

Functional expression of phosphagen kinase systems confers resistance to transient stresses in Saccharomyces cerevisiae by buffering the ATP pool.

Authors: Canonaco Fabrizio; Schlattner Uwe; Pruett Pamela S; Wallimann Theo; Sauer Uwe;

Journal:J Biol Chem

PubMed ID:12036963

'Phosphagen kinase systems provide different advantages to tissues with high and fluctuating energy demands, in particular an efficient energy buffering system. In this study we show for the first time functional expression of two phosphagen kinase systems in Saccharomyces cerevisiae, which does not normally contain such systems. First, to establish ... More

Serinc, an activity-regulated protein family, incorporates serine into membrane lipid synthesis.

Authors:Inuzuka M, Hayakawa M, Ingi T,

Journal:J Biol Chem

PubMed ID:16120614

Cell membranes contain various transporter proteins, some of which are responsible for transferring amino acids across membrane. In this study, we report another class of carrier proteins, termed Serinc1-5, that incorporates a polar amino acid serine into membranes and facilitates the synthesis of two serine-derived lipids, phosphatidylserine and sphingolipids. Serinc ... More

A vertebrate fatty acid desaturase with Delta 5 and Delta 6 activities.

Authors: Hastings N; Agaba M; Tocher D R; Leaver M J; Dick J R; Sargent J R; Teale A J;

Journal:Proc Natl Acad Sci U S A

PubMed ID:11724940

Delta5 and Delta6 fatty acid desaturases are critical enzymes in the pathways for the biosynthesis of the polyunsaturated fatty acids arachidonic, eicosapentaenoic, and docosahexaenoic acids. They are encoded by distinct genes in mammals and Caenorhabditis elegans. This paper describes a cDNA isolated from zebrafish (Danio rerio) with high similarity to ... More