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Invitrogen™
pLenti6.3/V5™-TOPO™ TA Cloning™ Kit
Invitrogen's™ pLenti6.3⁄V5™-TOPO™Cloning Kit is part of our ViraPower™ HiPerform™ Lentiviral Expression System (catalog K5310-00). The pLenti6.3⁄V5™-TOPO™ -TA Cloning Kit containsRead more
| Catalog Number | Quantity |
|---|---|
| K531520 also known as K5315-20 | 1 kit |
Catalog number K531520
also known as K5315-20
Price (CNY)
42,232.00
飞享价
Ends: 31-Dec-2026
49,391.00Save 7,159.00 (14%)
Each
Quantity:
1 kit
Price (CNY)
42,232.00
飞享价
Ends: 31-Dec-2026
49,391.00Save 7,159.00 (14%)
Each
Invitrogen's™ pLenti6.3⁄V5™-TOPO™Cloning Kit is part of our ViraPower™ HiPerform™ Lentiviral Expression System (catalog K5310-00).
The pLenti6.3⁄V5™-TOPO™ -TA Cloning Kit contains the TOPO™- adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5™-TOPO™ for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5™-TOPO™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• 5 minute, high-efficiency TOPO™ PCR cloning in three simple steps
Key Features
• HiPerform™ WPRE and cPPT elements
• CMV promoter
• V5 epitope tag at C terminus
• Blasticidin selection
Kit includes
• A lacZ vector as a positive control, pLenti6.3⁄V5-GW⁄lacZ™
• Stbl3™ competent cells
For research use only. Not intended for any therapeutic or diagnostic use.
The pLenti6.3⁄V5™-TOPO™ -TA Cloning Kit contains the TOPO™- adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5™-TOPO™ for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5™-TOPO™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• 5 minute, high-efficiency TOPO™ PCR cloning in three simple steps
Key Features
• HiPerform™ WPRE and cPPT elements
• CMV promoter
• V5 epitope tag at C terminus
• Blasticidin selection
Kit includes
• A lacZ vector as a positive control, pLenti6.3⁄V5-GW⁄lacZ™
• Stbl3™ competent cells
For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeCompetent Cell
Cloning MethodTOPO-TA
Constitutive or Inducible SystemConstitutive
Delivery TypeLentiviral
Expression SystemViraPower™ HiPerform™ Lentiviral Expression Systems
For Use With (Application)Protein Expression
Key FunctionsViral Expression
Product LineHiPerform, TOPO, ViraPower
Product TypeCloning Kit
PromoterCMV
Protein TagV5 Epitope Tag
Quantity1 kit
Selection Agent (Eukaryotic)Blasticidin
Selection Marker PromoterSV40 Promoter
VectorpLenti, TOPO-TA Vector
FormatKit
Unit SizeEach
Contents & Storage
TOPO™ TA Cloning Reagents (Box 1)
pLenti6.3?V5™-TOPO™ (5-10 ng⁄μL): 20 μL, -20°C
pLenti6.3⁄V5-GW⁄lacZ™ Control vector (0.5 μg⁄μL): 20 μL, -20°C
10X PCR Buffer: 100 μL, -20°C
dNTP Mix: 10 μL, -20°C
Salt Solution: 50 μL, -20°C
CMV Forward Primer (100 ng⁄μL): 20 μL, -20°C
V5 (C-term) Reverse Primer (100 ng⁄μL): 20 μL, -20°C
Control PCR Template (50 ng⁄μL): 10 μL, -20°C
Control PCR Primers (100 ng⁄μL): 10 μL, each -20°C
Sterile Water: 1 mL
One Shot™ Stbl3™ Chemically Competent E. coli (Box 2)
Stbl3™ Cells: 21 x 50 μL, -80°C
pUC19 control DNA (10 pg⁄μL): 50 μL, -80°C
S.O.C. Medium: 6 mL, -80°C
pLenti6.3?V5™-TOPO™ (5-10 ng⁄μL): 20 μL, -20°C
pLenti6.3⁄V5-GW⁄lacZ™ Control vector (0.5 μg⁄μL): 20 μL, -20°C
10X PCR Buffer: 100 μL, -20°C
dNTP Mix: 10 μL, -20°C
Salt Solution: 50 μL, -20°C
CMV Forward Primer (100 ng⁄μL): 20 μL, -20°C
V5 (C-term) Reverse Primer (100 ng⁄μL): 20 μL, -20°C
Control PCR Template (50 ng⁄μL): 10 μL, -20°C
Control PCR Primers (100 ng⁄μL): 10 μL, each -20°C
Sterile Water: 1 mL
One Shot™ Stbl3™ Chemically Competent E. coli (Box 2)
Stbl3™ Cells: 21 x 50 μL, -80°C
pUC19 control DNA (10 pg⁄μL): 50 μL, -80°C
S.O.C. Medium: 6 mL, -80°C