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Invitrogen™
pENTR™ 5'-TOPO™ TA Cloning™ Kit, with One Shot™ TOP10 Chemically Competent E. coli
The pENTR™ 5’-TOPO™ TA Cloning™ Kit combines Invitrogen’s TOPO™ Cloning and MultiSite Gateway™ Technologies to facilitate five-minute, one-step cloning ofRead more
| Catalog Number | Quantity |
|---|---|
| K59120 | 20 reactions |
Catalog number K59120
Price (CNY)
10,085.00
Each
Quantity:
20 reactions
Price (CNY)
10,085.00
Each
The pENTR™ 5’-TOPO™ TA Cloning™ Kit combines Invitrogen’s TOPO™ Cloning and MultiSite Gateway™ Technologies to facilitate five-minute, one-step cloning of a Taq polymerase-amplified PCR product encoding a eukaryotic promoter of interest into a MultiSite Gateway™ entry vector with ≥ 85% efficiency. Once cloned, your eukaryotic promoter may be transferred from the pENTR™ 5'-TOPO™ vector to a suitable MultiSite Gateway™ destination vector in a MultiSite Gateway™ LR recombination reaction with other suitable entry clone(s) to generate an expression construct.
This kit can be used in combination with ViraPower™ HiPerform Promoterless Lentiviral Gateway™ Vector Kit, or MultiSite Gateway™ Three-Fragment Vector Construction Kit.
Advantages
• Choose the best promoter for your experiments and cell line by efficiently expressing your gene of interest from multiple promoter constructs
• When using in conjunction with ViraPower™ HiPerform™ Promoterless Lentiviral vector, you can efficiently transduce and express your gene of interest driven by the promoter of choice in both dividing and non-dividing mammalian cells
Key Features
• Rapid PCR cloning with TOPO™ cloning technology for cloning the 5’ element
• attL4 & attR1 sites for compatibility with MultiSite Gateway™ LR reaction for increased flexibility
Kit Includes
• pENTR™ 5´-TOPO™ TA Cloning™ Kit
• One Shot™ TOP10 Chemically Competent E. coli (C4040-03)
Related SKUs
• ViraPower™ HiPerform™ Promoterless Lentiviral Gateway™ Expression System (A111451)
• ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit (A11146)
• MultiSite Gateway™ Three-Fragment Vector Construction Kit (12537-023)
For research use only. Not intended for any therapeutic or diagnostic use.
This kit can be used in combination with ViraPower™ HiPerform Promoterless Lentiviral Gateway™ Vector Kit, or MultiSite Gateway™ Three-Fragment Vector Construction Kit.
Advantages
• Choose the best promoter for your experiments and cell line by efficiently expressing your gene of interest from multiple promoter constructs
• When using in conjunction with ViraPower™ HiPerform™ Promoterless Lentiviral vector, you can efficiently transduce and express your gene of interest driven by the promoter of choice in both dividing and non-dividing mammalian cells
Key Features
• Rapid PCR cloning with TOPO™ cloning technology for cloning the 5’ element
• attL4 & attR1 sites for compatibility with MultiSite Gateway™ LR reaction for increased flexibility
Kit Includes
• pENTR™ 5´-TOPO™ TA Cloning™ Kit
• One Shot™ TOP10 Chemically Competent E. coli (C4040-03)
Related SKUs
• ViraPower™ HiPerform™ Promoterless Lentiviral Gateway™ Expression System (A111451)
• ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit (A11146)
• MultiSite Gateway™ Three-Fragment Vector Construction Kit (12537-023)
For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeTA Cloning Kit
Quantity20 reactions
VectorpENTR, TOPO-TA Cloning Vectors
Cloning MethodTOPO-TA
Product LineOne Shot
Unit SizeEach
Contents & Storage
The pENTR™ 5´-TOPO™ TA Cloning™ Kit with One Shot™ TOP10 Chemically Competent E. coli contains two boxes.
The TOPO™ Cloning box contains enough linearized and topoisomerase I-activated pENTR™ 5´-TOPO™ vector for 20 cloning reactions, dNTPs, salt solution, control template and primers and primers for sequencing. Store at -20°C.
The One Shot™ TOP10 box contains transformation reagents including 20 single-use 50 μl aliquots of One Shot™ Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C.
The TOPO™ Cloning box contains enough linearized and topoisomerase I-activated pENTR™ 5´-TOPO™ vector for 20 cloning reactions, dNTPs, salt solution, control template and primers and primers for sequencing. Store at -20°C.
The One Shot™ TOP10 box contains transformation reagents including 20 single-use 50 μl aliquots of One Shot™ Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C.