Ni-NTA Purification System

Citations & References (4)

Invitrogen™

Ni-NTA Purification System

The Ni-NTA Purification System is designed for the purification of recombinant proteins that contain a polyhistidine (6xHis) sequence. The kitRead more

Catalog Number	Quantity
K95001	6 purifications

Catalog number K95001

Price (CNY)

13,014.00

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Quantity:

6 purifications

Request bulk or custom format

Price (CNY)

13,014.00

Add to cart

The Ni-NTA Purification System is designed for the purification of recombinant proteins that contain a polyhistidine (6xHis) sequence. The kit utilizes Ni-NTA nickel-chelating resin and is supplied with native and denaturing buffers for efficient purification of recombinant proteins under different conditions.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

FormatSuspension

Product TypeNi-NTA Purification System

Quantity6 purifications

Protein TagHis Tag

Unit SizeEach

Contents & Storage

Six 2-ml resin columns and buffers for native and denaturing purification. Ten milliliters of Ni-NTA Agarose. Store at +4°C. All reagents are guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should the typical protein recovery be when using the Probond Purification System or Ni-NTA Purification system?

Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (4)

Citations & References

Abstract

A composite Ets/Pit-1 binding site in the prolactin gene can mediate transcriptional responses to multiple signal transduction pathways.

Authors:Howard PW, Maurer RA

Journal:J Biol Chem

PubMed ID:7673116

'Binding sites for the tissue-specific transcription factor, Pit-1, are required for basal and hormonally induced prolactin gene transcription. Although Pit-1 is phosphorylated in response to several signaling pathways, the mechanism by which Pit-1 contributes to hormonal induction of gene transcription has not been defined. Recent reports suggest that phosphorylation of ... More

Sulfation of N-acetylglucosamine by chondroitin 6-sulfotransferase 2 (GST-5).

Authors: Bhakta S; Bartes A; Bowman K G; Kao W M; Polsky I; Lee J K; Cook B N; Bruehl R E; Rosen S D; Bertozzi C R; Hemmerich S;

Journal:J Biol Chem

PubMed ID:10956661

'Based on sequence homology with a previously cloned human GlcNAc 6-O-sulfotransferase, we have identified an open reading frame (ORF) encoding a novel member of the Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST) family termed GST-5 on the human X chromosome (band Xp11). GST-5 has recently been characterized as a novel GalNAc 6-O-sulfotransferase termed chondroitin ... More

Probable Identification of a Membrane-Associated Repressor of Bacillus subtilis DNA Replication as the E2 Subunit of the Pyruvate Dehydrogenase Complex

Authors:Andrew Stein and William Firshein*

Journal:J Bacteriol

PubMed ID:10735853

Two Bacillus subtilis lysogenic libraries were probed by an antibody specific for apreviously described membrane-associated inhibitor of B. subtilis DNA replication(J. Laffan and W. Firshein, Proc. Natl. Acad. Sci. USA 85:7452-7456, 1988). Threeclones that reacted strongly with the antibody contained an entire open reading frame.Sequencing identified one of the clones ... More

Sox9 and p300 cooperatively regulate chromatin-mediated transcription.

Authors:Furumatsu T, Tsuda M, Yoshida K, Taniguchi N, Ito T, Hashimoto M, Ito T, Asahara H,

Journal:J Biol Chem

PubMed ID:16109717

Chromatin structure is a fundamental component of gene regulation, expression, and cellular differentiation. We have previously reported that the multifunctional coactivator p300 is a member of the Sox9-related transcriptional apparatus and activates Sox9 (Sry-type high-mobility-group box 9)-dependent transcription during chondrogenesis. However, the mechanism of synergy between Sox9 and p300 in ... More