LIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green
LIVE/DEAD&trade; Cell Vitality Assay Kit, C<sub>12</sub> Resazurin/SYTOX&trade; Green
Invitrogen™

LIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green

The LIVE/DEAD Cell Vitality Assay Kit provides a simple, two-color fluorescence assay that distinguishes metabolically active cells from injured cellsRead more
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Catalog NumberQuantity
L349511000 Assays kit
Catalog number L34951
Price (CNY)
4,325.00
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Ends: 31-Dec-2025
5,746.00
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Quantity:
1000 Assays kit
Price (CNY)
4,325.00
飞享价
Ends: 31-Dec-2025
5,746.00
Save 1,421.00 (25%)
Each
Add to cart
The LIVE/DEAD Cell Vitality Assay Kit provides a simple, two-color fluorescence assay that distinguishes metabolically active cells from injured cells and dead cells. The assay is based on the reduction of C12-resazurin to red-fluorescent C12-resorufin in metabolically active cells and on the uptake of the cell-impermeant, green-fluorescent nucleic acid stain, SYTOX Green dye, in cells with compromised plasma membranes (usually late apoptotic and necrotic cells). In this assay, dead cells emit mostly green fluorescence and healthy, metabolically active cells emit mostly red fluorescence; injured cells exhibit reduced red and green fluorescence.

View a selection guide for all Cell Viability Assays for Flow Cytometry.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityImpermeant, Permeant
Cell TypeMammalian Cells, Eukaryotic Cells
DescriptionLIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green
Detection MethodFluorescence
Dye TypeOther Label(s) or Dye(s)
FormatTube(s)
Quantity1000 Assays kit
Shipping ConditionRoom Temperature
SolubilityDMSO (Dimethylsulfoxide)
ColorGreen, Red
EmissionC12 Resazurin: 563/587
SYTOX™ Green: 504/523
Excitation Wavelength Range504/563 nm
For Use With (Application)Viability Assay
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Product LineLIVE/DEAD, SYTOX
Product TypeCell Vitality Assay Kit
Unit SizeEach
Contents & Storage
Contains 5 vials of C12-resazurin (40 μg each vial), 1 vial of SYTOX™ green (100 μL, 10 μM solution in DMSO), 1 vial of DMSO (1.5 mL), and 10X phosphate buffer (100 mL).

Store in freezer (-5°C to -30°C) and protect from light.

Frequently asked questions (FAQs)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do alamarBlue reagent and PrestoBlue reagent differ from resazurin and C12-resazurin?

alamarBlue reagent and PrestoBlue reagent contain resazurin in a proprietary stabilizing formulation that allows for a convenient “mix, incubate, and read” protocol. PrestoBlue reagent is an improvement in the formulation of alamarBlue reagent that allows for much faster staining (typically 10 minutes vs. 1-4 hours to obtain a similar signal and sensitivity). C12-resazurin is a derivative of resazurin that has better cellular retention and thus allows for analysis on a flow cytometer and multiplexing with viability indicators and other biomarkers.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.