NuPAGE™ Tris-Acetate SDS Running Buffer (20X)
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NuPAGE™ Tris-Acetate SDS Running Buffer (20X)
Invitrogen™

NuPAGE™ Tris-Acetate SDS Running Buffer (20X)

NuPAGE Tris-Acetate SDS Running Buffer (20X) is formulated for separation of proteins in their denatured state on Tris-Acetate gels. NuPAGERead more
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Catalog NumberQuantity
LA0041500 mL
Catalog number LA0041
Price (CNY)
658.00
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Ends: 31-Dec-2025
928.00
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Quantity:
500 mL
Price (CNY)
658.00
飞享价
Ends: 31-Dec-2025
928.00
Save 270.00 (29%)
Each
Add to cart
NuPAGE Tris-Acetate SDS Running Buffer (20X) is formulated for separation of proteins in their denatured state on Tris-Acetate gels. NuPAGE Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer. NuPAGE Tris-Acetate gels can also be run with Novex Tris-Glycine Native Running Buffer to resolve native proteins more effectively than with the Tris-Glycine gel system.

Recommended buffers
Run NuPAGE Tris-Acetate gels with NuPAGE Tris-Acetate SDS Running Buffer and to ensure good sample reduction and band resolution, use NuPAGE LDS Sample Buffer. For native running conditions, the Tris-Glycine Native running and sample buffers should be used.

See all available buffers and reagents available for SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferRunning Buffers
Concentration20 X
Gel CompatibilityNuPAGE™ Tris-Acetate Gels
Product LineNuPAGE™
Product TypeSDS Running Buffer
Quantity500 mL
Shelf Life1 Year
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Gel TypeSDS-PAGE
Unit SizeEach
Contents & Storage
Store at +4°C to 25°C. Guaranteed stable for 6 months unless otherwise specified in the product literature.

Frequently asked questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What are the recommended sample loading volumes and protein loading amounts for your precast protein gels?

*Tris-Glycine and Invitrogen Tricine Mini gels: see here (http://tools.thermofisher.com/content/sfs/manuals/electrophoresisguide_man.pdf), Page 8

*NuPAGE Tris-Acetate and NuPAGE Bis-Tris Mini gels: see here (http://tools.thermofisher.com/content/sfs/manuals/nupage_tech_man.pdf), Page 10

*Bolt Bis-Tris Plus Mini gels: see here (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/bolt-bis-tris-gels.html)

*Thermo Scientific Precise Tris-HEPES gels: see here (https://tools.thermofisher.com/content/sfs/manuals/MAN0011499_Precise_Protein_Gels_UG.pdf), Page 1

*Midi gels (Invitrogen Tris-Glycine, NuPAGE Bis-Tris and NuPAGE Tris-Acetate): see here (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/novex_midigel_man.pdf), Page 4

*Thermo Scientific Precise Tris-Glycine gels: see here (https://tools.thermofisher.com/content/sfs/manuals/D25MAN0011814_Precise_TrisGlycine_Gels_UG.pdf), Page 1

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Do your precast protein gels contain SDS?

Our precast protein gels do not contain SDS but they can be run under denaturing conditions when used with the appropriate denaturing running buffer.
Note: NuPAGE Bis-Tris gels, Bolt Bis-Tris Plus gels, and Thermo Scientific Precise Tris-HEPES gels cannot be run under native conditions; they can only be run under denaturing conditions.

*Invitrogen Tris-Glycine gels: For Native electrophoresis, use Invitrogen Tris-Glycine Native Running Buffer. For Denaturing electrophoresis, use Invitrogen Tris-Glycine SDS Running Buffer

*NuPAGE Tris-Acetate gels: For Native electrophoresis, use Invitrogen Tris-Glycine Native Running Buffer. For Denaturing electrophoresis, use NuPAGE Tris-Acetate SDS Running Buffer

*NuPAGE Bis-Tris gels: For Denaturing electrophoresis, use NuPAGE MOPS-SDS Running Buffer or NuPAGE MES-SDS Running Buffer

*Bolt Bis-Tris Plus gels: For Denaturing electrophoresis, use Bolt MOPS SDS Running Buffer or Bolt MES SDS Running Buffer

*Thermo Scientific Precise Tris-Glycine gels: For Native electrophoresis, use Tris-Glycine SDS Running Buffer without SDS added. For Denaturing electrophoresis, use Tris-Glycine SDS Running Buffer.

*Thermo Scientific Precise Tris-HEPES gels: For Denaturing electrophoresis, use Tris-HEPES SDS Running Buffer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.