NeuroTrace™ Multicolor Tissue-Labeling Kit - DiO, DiI, DiD pastes, 500 mg each
NeuroTrace™ Multicolor Tissue-Labeling Kit - DiO, DiI, DiD pastes, 500 mg each
Invitrogen™

NeuroTrace™ Multicolor Tissue-Labeling Kit - DiO, DiI, DiD pastes, 500 mg each

NeuroTrace™ Multicolor Tissue-Labeling Kit contains 500 mg each of DiI, DiO or DiD mixed into an inert, water-resistant gel. TheRead more
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Catalog NumberQuantity
N228841 kit
Catalog number N22884
Price (CNY)
8,911.00
Each
Add to cart
Quantity:
1 kit
Price (CNY)
8,911.00
Each
Add to cart
NeuroTrace™ Multicolor Tissue-Labeling Kit contains 500 mg each of DiI, DiO or DiD mixed into an inert, water-resistant gel. The paste is ready to use as supplied and can be applied directly to live or fixed tissue specimens using the tip of a needle. This method of application improves the penetration of the dye into bundled neurons, labeling axons both on and below the surface.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorGreen, Yellow, Red
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope
Product TypeTissue Labeling Kit
Quantity1 kit
Shipping ConditionRoom Temperature
Sub Cellular LocalizationCell Membranes & Lipids
Product LineNEUROTRACE™
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.

Frequently asked questions (FAQs)

I am doing retrograde neuronal labeling using the NeuroTrace DiI Paste. I apply a small glob onto the neuron of interest, but it is not adhering very well. What can I do?

This paste, which has DiI C18 uniformly dispersed within a proprietary hydrophobic carrier, is commonly used to dab onto larger areas than smaller areas commonly used with crystals. Any moisture on the area the paste is applied may be the cause for lack of adherence. Apply the paste using a small flat-head spatula or toothpick and keep it in place a bit longer to ensure contact. The area may also be covered with parafilm to ensure contact as well.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What products do you have for neuronal tracing?

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.