NanoOrange™ Protein Quantitation Kit, 200-2,000 assays
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NanoOrange™ Protein Quantitation Kit, 200-2,000 assays
NanoOrange™ Protein Quantitation Kit, 200-2,000 assays
NanoOrange™ Protein Quantitation Kit, 200-2,000 assays
NanoOrange™ Protein Quantitation Kit, 200-2,000 assays
Citations & References (29)
Invitrogen™

NanoOrange™ Protein Quantitation Kit, 200-2,000 assays

The NanoOrange Protein Quantitation Kit contains a very sensitive and easy assay for protein quantitation, with detection as low asRead more
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Catalog NumberQuantity
N66661 Kit
Catalog number N6666
Price (CNY)
1,974.00
Each
Add to cart
Quantity:
1 Kit
Request bulk or custom format
Price (CNY)
1,974.00
Each
Add to cart
The NanoOrange Protein Quantitation Kit contains a very sensitive and easy assay for protein quantitation, with detection as low as 10 ng/mL of protein in solution. This fluorescent dye is suitable for use with spectrofluorometers and microplate readers. For detection of lipoproteins or proteins in a complex lipid environment, check out our CBQCA Protein Quantitation Kit (C-6667).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AssayFluorescent Protein Assay
For Use With (Application)Protein quantitation
For Use With (Equipment)Microplate Reader
Product LineNanoOrange
Product TypeProtein Quantitation Assay
Quantity1 Kit
Sufficient For200 to 2000 Assays
Detection MethodFluorescence
Unit SizeEach

Frequently asked questions (FAQs)

When preparing the serial dilutions for the standard curve for the NanoOrange Protein Quantitation Kit (Cat. No. N6666), can they first be diluted in buffer (e.g., Tris) or do they have to be diluted in 1X NanoOrange working solution?

All samples and standards need to be diluted in 1X NanoOrange working solution, not in any other buffer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

My buffer or components of my buffer are not listed in the compatibility table for my protein assay. What should I do?

You can test the tolerance of the assay for your specific buffer formulation. For in-house generated compatibility information, substances were considered compatible at the indicated concentration in the Standard Test Tube Protocol (found in the manual for each protein assay) if the error in protein concentration estimation caused by the presence of the substance was less than or equal to 10%. The substances were tested using WR prepared immediately before each experiment. Blank-corrected 562nm absorbance measurements (for a 1000µg/mL BSA standard + substance) were compared to the net 562nm measurements of the same standard prepared in 0.9% saline.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

All the components of my sample buffer are at or below the indicated compatible concentration for my protein assay, but I am still seeing too much/too little color development. What could be the problem?

It is possible to have a substance additive affect such that even though a single component is present at a concentration below its listed compatibility, a sample buffer containing a combination of substances could interfere with the assay. You should take steps to eliminate or minimize the effects of the interfering substance(s) by diluting or removing the substance.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

My protein assay is not developing color or is developing too much color. What can I do?

Refer to the information in the product-specific instruction booklet or our Tech Tip: Protein Quantitation Assay Compatibility Table (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0068-Protein-assay-compatibility.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

My spectrophotometer doesn’t have a filter set for the absorbance maximum. Can I use an alternate wavelength to read the protein assay?

Often, an alternative wavelength can be used, although the slope of the standard curve and the overall assay sensitivity will most likely be reduced. Our Tech Tip (https://tools.thermofisher.com/content/sfs/brochures/TR0025-Protein-assay-spectra.pdf) offers additional information on determining acceptable wavelengths for measuring protein assays.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all NanoOrange™ Protein Quantitation Kit, 200-2,000 assays FAQs

Citations & References (29)

Citations & References
Abstract
Authors:
Journal:
PubMed ID:10652312
Identification of cyclic AMP-regulated genes in Mycobacterium tuberculosis complex bacteria under low-oxygen conditions.
Authors:Gazdik MA, McDonough KA
Journal:J Bacteriol
PubMed ID:15805514
'Mycobacterium tuberculosis is the etiological agent of tuberculosis (TB), which kills approximately 2 million people a year despite current treatment options. A greater understanding of the biology of this bacterium is needed to better combat TB disease. The M. tuberculosis genome encodes as many as 15 adenylate cyclases, suggesting that ... More
Regulation of collagen deposition and lysyl oxidase by tumor necrosis factor-alpha in osteoblasts.
Authors:Pischon N, Darbois LM, Palamakumbura AH, Kessler E, Trackman PC
Journal:J Biol Chem
PubMed ID:15138266
'Tumor necrosis factor-alpha (TNF-alpha) inhibits osteoblast function in vitro by inhibiting collagen deposition. Studies generally support that TNF-alpha does not inhibit collagen biosynthesis by osteoblasts but that collagen deposition is in some way diminished. The study investigated TNF-alpha regulation of biosynthetic enzymes and proteins crucial for posttranslational extracellular collagen maturation ... More
Control of neuronal size homeostasis by trophic factor-mediated coupling of protein degradation to protein synthesis.
Authors:Franklin JL, Johnson EM
Journal:J Cell Biol
PubMed ID:9732291
'We demonstrate that NGF couples the rate of degradation of long-lived proteins in sympathetic neurons to the rate of protein synthesis. Inhibiting protein synthesis rate by a specific percentage caused an almost equivalent percentage reduction in the degradation rate of long-lived proteins, indicating nearly 1:1 coupling between the two processes. ... More
Key role of conserved histidines in recombinant mouse beta-carotene 15,15'-monooxygenase-1 activity.
Authors:Poliakov E, Gentleman S, Cunningham FX, Miller-Ihli NJ, Redmond TM
Journal:J Biol Chem
PubMed ID:15951442
'Alignment of sequences of vertebrate beta-carotene 15,15''-monooxygenase-1 (BCMO1) and related oxygenases revealed four perfectly conserved histidines and five acidic residues (His172, His237, His308, His514, Asp52, Glu140, Glu314, Glu405, and Glu457 in mouse BCMO1). Because BCMO1 activity is iron-dependent, we propose that these residues participate in iron coordination and therefore are ... More
View all NanoOrange™ Protein Quantitation Kit, 200-2,000 assays citations