Newport Green™ DCF Diacetate, cell permeant
Newport Green™ DCF Diacetate, cell permeant
Citations & References (48)
Invitrogen™

Newport Green™ DCF Diacetate, cell permeant

The Newport Green™ DCF indicator has moderate zinc-binding affinity (Kd for Zn2+ ∼1 μM) but is essentially insensitive to Ca2+Read more
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Catalog NumberQuantity
N79911 mg
Catalog number N7991
Price (CNY)
3,768.00
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Ends: 31-Dec-2026
5,161.00
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Quantity:
1 mg
Price (CNY)
3,768.00
飞享价
Ends: 31-Dec-2026
5,161.00
Save 1,393.00 (27%)
Each
Add to cart

The Newport Green™ DCF indicator has moderate zinc-binding affinity (Kd for Zn2+ ∼1 μM) but is essentially insensitive to Ca2+ (Kd for Ca2+ >100 μM), making this a valuable probe for detecting Zn2+ influx into neurons through voltage- or glutamate-gated channels. When used alongside dyes with dual Ca2+ / Zn2+ sensitivity such as fura-2 and mag-fura-2, Newport Green™ DCF provides confirmation that changes in Zn2+ levels, and not Ca2+ or Mg2+, are being detected. The response of Newport Green™ DCF to transition metals, including Zn, Mn, Fe, Co, Cu(I), Cu(II), Ni and Cd, has been characterized.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

Newport Green™ PDX has a higher Zn2+ dissociation constant (Kd for Zn2+ ∼30 μM) and a larger Zn2+-free to Zn2+-saturated fluorescence intensity increase than the Newport Green™ DCF indicators.

Fluorescent Zinc Indicators Specifications:

  • Label (Ex/Em): Newport Green™ DCF (∼505/535 nm)
  • Kd (Zn2+) (in buffer): ∼1 μM
  • Lyophilized product may be dissolved in DMSO for use
  • Product is typically loaded into cells by adding the dissolved indicator to medium containing cells

Find Fluorescent Indicators for Zinc and Other Metal Ions

We offer a number of fluorescent indicators to determine polyvalent cation concentrations inside cells, to follow metal ion transport through ion channels, to make measurements in environmental samples, and more. Review Fluorescent Indicators for Zn2+ and Other Metal Ions—Section 19.7 in the Molecular Probes™ Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeZinc Indicator
Quantity1 mg
Shipping ConditionRoom Temperature
For Use With (Application)Cell Viability and Proliferation
For Use With (Equipment)Fluorescence Microscope
Product LineNewport Green
Product TypeDCF Diacetate
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C.

Citations & References (48)

Citations & References
Abstract
Calcineurin/nuclear factor of activated T cells and MAPK signaling induce TNF-{alpha} gene expression in pancreatic islet endocrine cells.
Authors:Lawrence MC, Naziruddin B, Levy MF, Jackson A, McGlynn K,
Journal:J Biol Chem
PubMed ID:21059644
Cytokines contribute to pancreatic islet inflammation, leading to impaired glucose homeostasis and diabetic diseases. A plethora of data shows that proinflammatory cytokines are produced in pancreatic islets by infiltrating mononuclear immune cells. Here, we show that pancreatic islet a cells and ß cells express tumor necrosis factor-a (TNF-a) and other ... More
Identification and purification of functional human beta-cells by a new specific zinc-fluorescent probe.
Authors:Lukowiak B, Vandewalle B, Riachy R, Kerr-Conte J, Gmyr V, Belaich S, Lefebvre J, Pattou F
Journal:J Histochem Cytochem
PubMed ID:11259455
'Pancreatic beta-cells contain large amounts of zinc. We took advantage of this to try to localize, quantify, and isolate insulin-producing cells from islet preparations. Our study was designed to identify a non-toxic zinc-sensitive fluorescent probe able to selectively label labile zinc in viable beta-cells and to exhibit excitation and emission ... More
Preferential Zn2+ influx through Ca2+-permeable AMPA/kainate channels triggers prolonged mitochondrial superoxide production.
Authors:Sensi SL, Yin HZ, Carriedo SG, Rao SS, Weiss JH
Journal:Proc Natl Acad Sci U S A
PubMed ID:10051656
'Synaptically released Zn2+ can enter and cause injury to postsynaptic neurons. Microfluorimetric studies using the Zn2+-sensitive probe, Newport green, examined levels of [Zn2+]i attained in cultured cortical neurons on exposure to N-methyl-D-asparte, kainate, or high K+ (to activate voltage-sensitive Ca2+ channels) in the presence of 300 microM Zn2+. Indicating particularly ... More
Measurement of intracellular free zinc in living cortical neurons: routes of entry.
Authors:Sensi SL, Canzoniero LM, Yu SP, Ying HS, Koh JY, Kerchner GA, Choi DW
Journal:J Neurosci
PubMed ID:9391010
'We used the ratioable fluorescent dye mag-fura-5 to measure intracellular free Zn2+ ([Zn2+]i) in cultured neocortical neurons exposed to neurotoxic concentrations of Zn2+ in concert with depolarization or glutamate receptor activation and identified four routes of Zn2+ entry. Neurons exposed to extracellular Zn2+ plus high K+ responded with a peak ... More
Protection by glycine against hypoxic injury of rat hepatocytes: inhibition of ion fluxes through nonspecific leaks.
Authors:Frank A, Rauen U, de Groot H
Journal:J Hepatol
PubMed ID:10673068
'BACKGROUND/AIMS: Glycine has long been shown to exert strong protective effects against hypoxic injury of hepatocytes. Recently, it was suggested that glycine exerts this protection via inhibition of ligand-gated chloride channels, thereby secondarily inhibiting sodium influx. The purpose of this study was to examine this suggestion. METHODS: Cultured rat hepatocytes ... More
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