AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.
Features of this enzyme:
AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for PCR, a testimony to its overall utility and efficacy
Its thermal activity profile makes it reliable for PCR applications
It is QC-tested to guarantee reproducible results
Reliable and robust PCR The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55°C–75°C). The enzyme's half-life is ∼40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. AmpliTaq DNA Polymerase is supplied with GeneAmp10X PCR Buffer I. It is also available with GeneAmp 10X PCR Buffer II and MgCl2 Solution.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection Method
Primer-probe
Format
Stand-alone enzyme
Polymerase
AmpliTaq DNA Polymerase
Reaction Speed
Standard
Exonuclease Activity
5' - 3'
Product Line
AmpliTaq™
Product Type
DNA Polymerase
Quantity
3000 Units
Shipping Condition
Dry Ice
Concentration
5 U/μL, 10X
Fidelity (vs. Taq)
1 X
Hot Start
No
No. of Reactions
2400
Overhang
3'-A
Reaction Format
Separate Components
Size (Final Product)
5 kb or less
Starting Material
DNA
Contents & Storage
Contents: • 12 × 50 μL each of 5 U/μL of AmpliTaq™ DNA Polymerase • 12 × 1.5 mL of GeneAmp™ 10X PCR Buffer (100 mM Tris-HCl pH 8.3, 500 mM KCl, 15 mM MgCl2, 0.01% (w/v) gelatin).