How are the Neon NxT Electroporation System 1-Channel Tubes (Cat. No. NT24 and NT96) packaged?
The tubes are packaged in groups of 4. Therefore, Cat. No. NT24 will have 6 bags of 4 tubes and Cat. No. NT96 will have 24 bags of 4 tubes.
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What state should the cells be in for best results with the Neon Nxt Electoporation System?
For best results with the Neon Nxt Electoporation System, the cells should be healthy (not stressed) and actively dividing so that the nuclear membrane integrity is already weakened for payload needing to enter the nucleus. Consider starving the cells of serum or other essential nutrients.
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I'm experiencing arcing issues with the Neon Nxt Electoporation System. What could be causing this?
Arcing could be caused by high voltage or pulse length settings, high salt or contaminants in the DNA sample, incorrect cell density, and air bubbles formed during mixing of cell samples. Mix samples gently to avoid air bubble formation and pipette samples in a slow, smooth motion to avoid air uptake.
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After electroporation using the Neon NxT Electroporation System, how long should I wait before analyzing protein expression?
The optimal time for analysis of protein expression is dependent upon the stability of the protein being expressed. For a short-lived protein, like luciferase, analysis of protein expression should be done at 6-18 hours after electroporation. For a more stable protein, such as GFP, you should start the analysis at 24 hours or longer post-electroporation.
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What is the best time for analysis of CRISPR/Cas9-based KO/KI application in T cells after electroporation using the Neon NxT Electroporation System?
We recommend analyzing the cells 72 hours post-electroporation.
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