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Citations & References (32)
Invitrogen™
1-pyrenebutanoic acid, succinimidyl ester
1-pyrenebutanoic acid, succinimidyl ester
| Catalog Number | Quantity |
|---|---|
| P130 | 100 mg |
Catalog number P130
Price (CNY)
5,199.00
Each
Quantity:
100 mg
Price (CNY)
5,199.00
Each
The amine-reactive 1-pyrenebutanoic acid, succinimidyl ester can be used to create environment-sensitive bioconjugates with this unique fluorophore.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine
Label or DyePyrene
Product Type1-Pyrenebutanoic Acid Succinimidyl Ester
Quantity100 mg
Reactive MoietyActive Ester, Succinimidyl Ester
Shipping ConditionRoom Temperature
Label TypeClassic Dyes
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
Citations & References (32)
Citations & References
Abstract
RNA tertiary folding monitored by fluorescence of covalently attached pyrene.
Journal:Biochemistry
PubMed ID:10571996
'The pathways by which large RNAs adopt tertiary structure are just beginning to be explored, and new methods that reveal RNA folding are highly desirable. Here we report an assay for RNA tertiary folding in which the fluorescence of a covalently incorporated chromophore is monitored. Folding of the 160-nucleotide Tetrahymena
Effects of Mg2+ and the 2' OH of guanosine on steps required for substrate binding and reactivity with the Tetrahymena ribozyme reveal several local folding transitions.
Journal:Biochemistry
PubMed ID:9287156
'Transient kinetic studies with fluorescence detection were used to determine the effects of Mg2+ concentration and the 2' OH group of guanosine monophosphate, prG, substrate on various steps in the transesterification reaction of prG with 5' pyrene-labeled oligonucleotides as catalyzed by the L-21 ScaI ribozyme. The effect of increasing Mg2+
Human plasma fibronectin structure probed by steady-state fluorescence polarization: evidence for a rigid oblate structure.
Journal:Biochemistry
PubMed ID:2337580
'In order to more clearly define the structure of human plasma fibronectin (PFn) under physiologic buffer conditions, we determined the mean harmonic rotational relaxation times (rho H) of PFn and the thrombin-derived 190/170-kDa PFn fragment using steady-state fluorescence polarization. These measurements utilized the long lifetime emission (tau = 1.2 X
Spontaneous and plasma factor-mediated transfer of pyrenyl cerebrosides between model and native lipoproteins.
Journal:Biochim Biophys Acta
PubMed ID:3931685
'A series of pyrenyl glucocerebrosides was synthesized by reacylation of psychosine with pyrene-labeled fatty acids having 3-11 methylene units. When incorporated into model high-density lipoproteins consisting of dimyristoylphosphatidylcholine-apolipoprotein A-II complexes and incubated with unlabeled complexes, these lipids exhibited spontaneous transfer. Half times of transfer varied from 1.5 min to 365
Productive and nonproductive intermediates in the folding of denatured rhodanese.
Journal:J Biol Chem
PubMed ID:10617586
'The competition between protein aggregation and folding has been investigated using rhodanese (thiosulfate:cyanide sulfurtransferase, EC 2.8.1.1) as a model. During folding from a urea-denatured state, rhodanese rapidly forms associated species or intermediates, some of which are large and/or sticky. The early removal of such particles by filtration results in a